1. M.Jabari 2005

2. Pipette Graduated (Measuring ) To deliver (EX) To contain (IN) (Wash with solution ) I Line II Line Volumetric

3. % CV must be less than 0.3 % % Bias must be less than 0.6% Ideal % Bias 0 – 0.6% is acceptable

4. Expected value Observed value _ % Bias = X 100  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD

5. GRAVIMETRY METHOD COLORIMETRY METHOD

6.  First weight a dish.  Then with a pipette that you want caliber it transfer DW into the dish and weight that.  Repeat 10 time this action and record weight.  Caculate each time pure weight of DW that transfer with pipette.  Caculate SD, %CV and % Biass.

7. 0.00160.0410.10 1 0.00810.0910.15 2 0.03610.1910.25 3 0.00360.0610.12 4 0010.06 5 0.00090.0310.09 6 0.0441-0. 219.85 7 0.00010.0110.07 8 0.0049-0.079.99 9 0.0196-0149.91 10 (X – mean) 2 Mean = 10.06  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD 0.1191.0710.06  (x- M ) 2 = 0.119 = 0.16= 0.4 = 0.04 Acceptable 0.04 < 0. 3

8. Mean of Expected weight Mean of Observed weight _ % Bias = X 100 0.00160.0410.10 1 0.00810.0910.15 2 0.03610.1910.25 3 0.00360.0610.12 4 0010.06 5 0.00090.0310.09 6 0.0441-0. 219.85 7 0.00010.0110.07 8 0.0049-0.079.99 9 0.0196-0149.91 10 (X – mean) 2 0.1191.0710.06 10 10.06 % Bias = X 100 _ = 0.006 _ Acceptable 0.006 < 0. 6

9. Methyl orange 492 nm Phenol red 405 nm

10.  For 1 ml pipette :  In a ballon class A.  Prepare 0.1 gr /100 ml (stock ).  Dilute 1 : 11 and measure absorbance.  Select 10 tube and add 10 ml DW in each tube. (for 2 ml add 20 ml DW )  Add 1 ml stock reagent into each tube.  Measur absorbance and record it.

11. Mean of Expected OD Mean of Observed OD _ % Bias = X 100 442 435 % Bias = X 100 _ = 1.58 _ 100-10425 1 255440 2 16-4431 3 25-5430 4 49-7428 5 366441 6 00435 7 255440 8 819444 9 11436 10 (X – mean) 2 3580435 Absorbance of 1:11 in class A ballone is 442 1.58 > 0.6 No acceptable

12. Mean = 435  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = 358 = 39= 6.2 = 1.4 100-10425 1 255440 2 16-4431 3 25-5430 4 49-7428 5 366441 6 00435 7 255440 8 819444 9 11436 10 (X – mean) 2 3580435 1.4 > 0.3 No acceptable

13. GRAVIMETRY METHOD COLORIMETRY METHOD

14.  Each sampler shoud assese 3 – 4 time annually.  Acceptable % CV up to 3%  Acceptable % Biass up to 5%

15.  First weight a dish.  Then with a sampler that you want caliber it transfer DW into the dish and weight that.  Repeat 10 time this action and record weight.  Caculate each time pure weight of DW that transfer with sampler.  Caculate SD, %CV and % Biass.

16. 0.49-0.7100 1 1.691.3102 2 1.691.3102 3 5.292.3103 4 2.89-1.799 5 2.89-1.799 6 0.090.3101 7 0.49-0.7100 8 0.0903101 9 0.49-0.7100 10 (X – mean) 2 Mean = 100.7  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD 16.1100.7  (x- M ) 2 = 16.1 = 1.78= 1.33 = 1.3 1.3 < 3 Acceptable

17. Mean of Expected weight Mean of Observed weight % Bias = X 100 100 100.7 % Bias = X 100 _ = 0.7 _ 0.49-0.7100 1 1.691.3102 2 1.691.3102 3 5.292.3103 4 2.89-1.799 5 2.89-1.799 6 0.090.3101 7 0.49-0.7100 8 0.0903101 9 0.49-0.7100 10 (X – mean) 2 16.1100.7 0.7 < 5 Acceptable

18. Methyl orange 492 nm Phenol red 405 nm

19. llll  For 10-100 ml sampler :  In a ballon class A.  Prepare 0.1 gr /100 ml (stock ).  Dilute 1 : 101 and measure absorbance.  Select 10 tube and add 1 ml DW in each tube. ( for 20  l,50  l,100  l add 2, 5, 10 ml DW )  Add 10  l stock reagent into each tube.  Measur absorbance and record it.

20. Mean of Expected OD Mean of Expected weight Mean of Observed OD _ % Bias = X 100 455 458.4 % Bias = X 100 _ = 0.74 _ 2.561.6460 1 6.762.6461 2 0.360.6459 3 0.16-0.4458 4 11.56-3.4455 5 0.16-0.4458 6 0.360.6459 7 2.561.6460 8 0.360.6459 9 11.56-3.4455 10 (X – mean) 2 36.40458.4 Absorbance of 1:101 in class A ballone is 455 0.74 < 5 Acceptable

21. Mean = 458.4  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = 36.4 = 4.04= 2.01 = 0.43 2.561.6460 1 6.762.6461 2 0.360.6459 3 0.16-0.4458 4 11.56-3.4455 5 0.16-0.4458 6 0.360.6459 7 2.561.6460 8 0.360.6459 9 11.56-3.4455 10 (X – mean) 2 36.40458.4 0.43 < 3 Acceptable

22. llll  For more than 100 ml sampler :  In a ballon class A.  Dilute 1 : 11 from stock reagent.