1. Scrolling clicker attendance See if you can figure out how to “click-in” on the scrolling screen to alert the computer that you are here.

2. Announcements i>clicker: now start to gain ec points Contracts? Stand up if completed reading for today (5-6 pages of textbook on Sickle-cell disease) Remain standing if took notes…

3. Thinking of what you want to get out of your college education and this course, which of the following is most important to you? a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

4. What you want to learn

5. Of these three goals, which one do you think you can make headway on outside of class by your own reading and studying? a)Acquiring information (facts, principles, concepts) b)Learning how to use information and knowledge in new situations c)Developing lifelong learning skills

6. You can do outside of class

7. Outside class ①Read- the assigned pages (in textbook) ②Write- notes/Highlight provided notes ③Review- Notes prior to start of lecture

8. Outside class ①Read- the assigned pages (in textbook) ②Write- notes/Highlight provided notes ③Review- Notes prior to start of lecture During lecture Review & solve problems- focus part of topic Annotate - your notes/Highlighted notes Bring questions- ask Qs you encountered

9. Extreme opportunities Readings not full or multiple chapters 1 lab experiment, 1 paper, 4 people Verbal Final –optional, if don’t pass take written Clickers usually ½ participation, ½ extra credit Resume is fat! Confidence high, after lab work. Course average grade ~85%

10. Syllabus & Contract Review contract and syllabus: If you accept this plan for the course, and your responsibilities, submit a signed copy to me.

13. A.. B.. C..

15. So what do you predict is the problem?

16. A. The deletion alters gating, thus blocking the CFTR. B. The deletion alters ATP binding, thus stopping CFTR. C. The deletion alters CFTR folding & cell throws it out. D. None of the above cause the disease.

17. biosynthesis of normal wild-type CFTR

18. biosynthesis of mutant ΔF508 CFTR PROTEASOME

19. “Correctors” goal help mutant ΔF508-CFTR

20. How many CFTR channels are on the surface of a cell of a CF patient? [If normal=100] A. 50 B. 25 C. 10 D. 5 E. 0

21. Benefits of ΔF508 mutation? The ΔF508 mutation most likely occurred over 50,000 years ago in Northern Europe. Individuals with two copies of ΔF508 get cystic fibrosis and often cannot reproduce. Yet having one copy of ΔF508 reduces water loss during cholera, greatly increasing the chance of survival.