1. Basics of Transfusion Therapy Laura Cooling MD, MS Associate Medical Director Transfusion Medicine

2. Blood Transfusion Policies and Standard Practices www.pathology.med.umich.edu Select: Clinical Resource “Blood Bank and Transfusion Services” Link on UMHS Clinical Homepage

3. Practical Guide to Transfusion Medicine By Marian Petrides, Gary Stack AABB Press PRINT RESOURCE:

4. THINK PINK PINK TOP TUBE FOR BLOOD BANK SPECIMENS ONLY

5. Blood Collection Most components results of single whole blood donations 500 mL blood + anticoagulant 20-30 min Blood processed into components –packed RBC, FFP, platelets, cryo –Manufactured Components: IVIgG, albumin, factor concentrates, etc….

6. Anticoagulants-Preservative Anticoagulant: Sodium citrate –prevent coagulation through chelation Ca++ Carbohydrate: Dextrose –RBC utilize glycolytic pathway Buffer: Na Phosphate –Counter lactic acid acumulation Adenine: Support ATP production Mannitol: Stabilize RBC membrane

8. ABO TYPES Fucose  1-2Galactose-R H-Antigen GalNAc  1-3Gal-R Fuc  1 2 A-Antigen Gal  1-3Gal-R Fuc  1 2 B-Antigen

9. DISTRIBUTION AND COMPATIBILITY OF ABO TYPES AND BLOOD COMPONENTS (holds true for solid organ transplant as well)

10. % Donors Compatible/Component