1. Physiology of red blood cells. Erythron. Respiratory pigments

2. Blood system Blood system firstly was proposed by Lung in 1936. Blood system firstly was proposed by Lung in 1936. It consist of It consist of - blood circulated through the blood circulatory system - blood circulated through the blood circulatory system - blood forming organs - blood forming organs - blood destroying organs - blood destroying organs - regulatory apparatus. - regulatory apparatus.

3. Blood Blood is a fluid connective tissue. Blood consist of Blood consist of - plasma - plasma - blood cells: - blood cells: 1. erythrocytes, 2. leucocytes, 3. platelets.

4. Function of blood

5. Functions of blood 1. Breathing function of blood. 1. Breathing function of blood. 2. Trophic function of blood. 2. Trophic function of blood. 3. Excretory function of blood. 3. Excretory function of blood. 4. Hormonal regulation. 4. Hormonal regulation. 6. Temperature regulation. 6. Temperature regulation. 7. Maintaining the acid-base balance of tissues. 7. Maintaining the acid-base balance of tissues. 8. Supporting the water-electrolytic balance. 8. Supporting the water-electrolytic balance. 9. Homeostasis function. 9. Homeostasis function. 10. Protecting the body from bacteria and other organisms that can cause diseases or other abnormal conditions. 10. Protecting the body from bacteria and other organisms that can cause diseases or other abnormal conditions.

6. Functions of Blood Transport of: Transport of: –Gases, nutrients, waste products –Processed molecules –Regulatory molecules Regulation of pH and osmosis Regulation of pH and osmosis Maintenance of body temperature Maintenance of body temperature Protection against foreign substances Protection against foreign substances Clot formation Clot formation

7. Production of Erythrocytes: Erythropoiesis

8. Quantity (volume) of blood, the concept of its deposit In adult in normal blood, relative to total body weight, is 6.8%. Newborns - 15%. Part of the blood is in the depot: liver - 20%, in the skin, subcutaneous vascular plexus - up to 10%, in the spleen - up to 1.5- 2% of blood. In adult in normal blood, relative to total body weight, is 6.8%. Newborns - 15%. Part of the blood is in the depot: liver - 20%, in the skin, subcutaneous vascular plexus - up to 10%, in the spleen - up to 1.5- 2% of blood. Deposited blood in comparison with blood circulating in the vessels is 10-20 times slower than blood cells. Deposited blood in comparison with blood circulating in the vessels is 10-20 times slower than blood cells..

9. Human blood smear X 500

10. Quantity of cells, their changing Erythrocytes In men – 4,0-5,1 Tera/L; in women – 3,7-4,7 Tera/L. The quantity of erythrocytes may be increase – in pregnancy, in physical training, mental work, in newborn or decrease. Leukocytes - 4-9 Giga/L. The number of leukocytes can increase – physical work, emotional load, in newborn, inflammation or decrease. Platelates - 180-320 Giga/L.

11. Blood cells

12. Plasma Water – 90 % Water – 90 % Solids – 10 % Solids – 10 % Inorganic chemicals: Inorganic chemicals: sodium, calcium, potassium, magnesium, chloride, bicarbonate, phosphate, sulfate – 0,9 % sodium, calcium, potassium, magnesium, chloride, bicarbonate, phosphate, sulfate – 0,9 % Organic chemicals: Organic chemicals: Proteins: serum albumin, serum globulin, fibrinogen – 8 % Proteins: serum albumin, serum globulin, fibrinogen – 8 % Others: – 1,1 % Others: – 1,1 % Nonprotein nitrogenous substances: urea, uric acid, creatine, creatinine, ammonium salts, amino acids Nonprotein nitrogenous substances: urea, uric acid, creatine, creatinine, ammonium salts, amino acids Nonnitrogenous substances: glucose, fats, cholesterol hormones Nonnitrogenous substances: glucose, fats, cholesterol hormones Gases: oxygen, carbon dioxide, nitrogen Gases: oxygen, carbon dioxide, nitrogen

13. Components of Whole Blood Withdraw blood and place in tube 1 2 Centrifuge Plasma (55% of whole blood) Formed elements Buffy coat: leukocyctes and platelets (<1% of whole blood) Erythrocytes (45% of whole blood) Hematocrit Males: 40-48% Females: 36- 42%

14. Proteins One liter of plasma has 65-85 gram of proteins. One liter of plasma has 65-85 gram of proteins. Concentration of Concentration of albumins is 35-50 g/L; albumins is 35-50 g/L; globulins is alpha-1-globulins – 1-4 g/L, globulins is alpha-1-globulins – 1-4 g/L, alpha-2-globulins – 4-8 g/L, alpha-2-globulins – 4-8 g/L, beta-globulins – 6-12 g/L, beta-globulins – 6-12 g/L, gamma-globulins – 8-16 g/L; gamma-globulins – 8-16 g/L; fibrinogen – 2-4 g/L. fibrinogen – 2-4 g/L. Plasma which are not contain fibrinogen called serum (it is necessary for understanding the immunology, therapy etc.) Plasma which are not contain fibrinogen called serum (it is necessary for understanding the immunology, therapy etc.)

15. Functional significance of plasma proteins Albumin: 80% defines oncotic pressure transfer bilirubin, urobilin, fatty acids, antibiotics, sulfonamides, are formed in the liver, 17 g per day. Albumin: 80% defines oncotic pressure transfer bilirubin, urobilin, fatty acids, antibiotics, sulfonamides, are formed in the liver, 17 g per day. Globulin. In a fraction of α1- globulins are proteins associated with carbohydrates. The fraction of α2- globulins include protein ceruloplasmin, thyroxine binding protein, vitamin B12 -binding globulin, angiotensin. By β- globulins include transfer of lipids, polysaccharides and iron. Globulin. In a fraction of α1- globulins are proteins associated with carbohydrates. The fraction of α2- globulins include protein ceruloplasmin, thyroxine binding protein, vitamin B12 -binding globulin, angiotensin. By β- globulins include transfer of lipids, polysaccharides and iron. Antibodies are mainly γ- globulins. Globulins are synthesized in the liver, bone marrow, spleen and lymph nodes. By day 5 grams of globulin synthesized Antibodies are mainly γ- globulins. Globulins are synthesized in the liver, bone marrow, spleen and lymph nodes. By day 5 grams of globulin synthesized Fibrinogen. (2-4 g / L) takes part in the formation of a blood clot. Formed exclusively in the liver. Fibrinogen. (2-4 g / L) takes part in the formation of a blood clot. Formed exclusively in the liver.

16. Buffer systems of blood Maintaining a constant acid-base balance of blood provided by buffer systems: Maintaining a constant acid-base balance of blood provided by buffer systems: 1. Bicarbonate buffer: Н 2 СО 3 + ОН – ⇄ НСО 3 – + Н 2 О, НСО 3 – + Н + ⇄ Н 2 СО 3 2. Phosphate buffer: Н 2 РО 4 – + ОН – ⇄ НРО 4 2– + Н 2 О, НРО 4 2– + Н + ⇄ Н 2 О 4 –

17. 3. The protein buffer: RСООН + ОН – ⇄ RООС – + Н 2 О, RСОО – + Н + ⇄ RООН RСОО – + Н + ⇄ RООН 4. Hemoglobin buffer. Essentially, there are two hemoglobin buffers - one based on reduced hemoglobin: NNb/Nb- and the other based on oxyhemoglobin: NHbO2/HbO2. First prevails in the venous blood, and the second - in the arterial: НHb + ОН – ⇄ Нb – + Н 2 О; НHbО 2 + ОН – ⇄ НbО 2 – + Н 2 О, Нb – + Н + ⇄ ННb; НbО 2 – + Н + ⇄ ННbО 2

18. Evaluation of acid-base balance is performed on the following parameters: 1. pH (from Eng. power Hydrogen - the power of hydrogen), which is equal to 7,35-7,45. 2. Tension of CO2 - pCO2, which is normally 5,3-6,1 kPa (40- 46 mm Hg). 3. Standard bicarbonate, international designation SB (standart bikarbonate) - estimated. At standard conditions is 20-27 mg / dL. 4. True, actual bicarbonate, international designation AB (actual bikarbonate), is 190-25 mg / dL. 5. Excess (deficiency) framework, international designation CE (D) (base ecxess (deficit) equals ± 2,3 mmol / l. 6. The sum of the bases of all the blood buffer systems, international designation BB (batter bases) is 40-60 mg / dL. 1. pH (from Eng. power Hydrogen - the power of hydrogen), which is equal to 7,35-7,45. 2. Tension of CO2 - pCO2, which is normally 5,3-6,1 kPa (40- 46 mm Hg). 3. Standard bicarbonate, international designation SB (standart bikarbonate) - estimated. At standard conditions is 20-27 mg / dL. 4. True, actual bicarbonate, international designation AB (actual bikarbonate), is 190-25 mg / dL. 5. Excess (deficiency) framework, international designation CE (D) (base ecxess (deficit) equals ± 2,3 mmol / l. 6. The sum of the bases of all the blood buffer systems, international designation BB (batter bases) is 40-60 mg / dL.

19. Destruction of red blood cells

20. The destruction of hemoglobin

21. EXCHANGE IRON IN THE BODY The basic amount of iron in the body (57.6%) is part of hemoglobin in red blood cells and is found. The basic amount of iron in the body (57.6%) is part of hemoglobin in red blood cells and is found. Iron enters the body in the food (meat, liver, fish, rice, peas, raisins, apricots). It is best absorbed iron, which is part of the food in the form of heme. Iron enters the body in the food (meat, liver, fish, rice, peas, raisins, apricots). It is best absorbed iron, which is part of the food in the form of heme.

22. The functions of red blood cells Transport function. Red blood cells carry: O2, CO2, NO, adsorbed proteins, drugs, physiologically active substances. Transport function. Red blood cells carry: O2, CO2, NO, adsorbed proteins, drugs, physiologically active substances. Provide acid-base balance. Provide acid-base balance. Maintaining the ionic composition of the plasma. Maintaining the ionic composition of the plasma. Hemostatic. Hemostatic.

23. The structure of erythrocyte Human erythrocytes - nuclear-cells that are shaped like concave discs 4,5-10,5 microns in diameter (average diameter of erythrocyte 7,55 m). With this form of erythrocyte, its surface is greater than it would be in spherical form. Human erythrocytes - nuclear-cells that are shaped like concave discs 4,5-10,5 microns in diameter (average diameter of erythrocyte 7,55 m). With this form of erythrocyte, its surface is greater than it would be in spherical form.

24. Scheme of hematopoiesis

25. Mechanisms regulation of erythropoiesis Regulation of erythropoiesis carried out by neural and humoral mechanisms, sympathetic innervation stimulates hematopoiesis and parasympathetic - brakes. Regulation of erythropoiesis carried out by neural and humoral mechanisms, sympathetic innervation stimulates hematopoiesis and parasympathetic - brakes. Of great importance is the regulation of erythropoiesis erythropoietin. Hematopoiesis is enhanced by hormones front of the pituitary gland, adrenal glands, thyroid gland. Male sex hormones stimulate, increasing the sensitivity of bone marrow to erythropoietin, and women - inhibit erythropoiesis. Of great importance is the regulation of erythropoiesis erythropoietin. Hematopoiesis is enhanced by hormones front of the pituitary gland, adrenal glands, thyroid gland. Male sex hormones stimulate, increasing the sensitivity of bone marrow to erythropoietin, and women - inhibit erythropoiesis. The body formed substances that inhibit erythropoiesis - inhibitors of erythropoiesis. Their content increases with the number of red blood cells, which do not meet the needs of the tissues for oxygen. Inhibitors of erythropoiesis lengthen cycle division erythroid cells, they inhibit the synthesis of hemoglobin. The body formed substances that inhibit erythropoiesis - inhibitors of erythropoiesis. Their content increases with the number of red blood cells, which do not meet the needs of the tissues for oxygen. Inhibitors of erythropoiesis lengthen cycle division erythroid cells, they inhibit the synthesis of hemoglobin.

26. Circulating erythrocytes – the number remains constant and reflects a balance between RBC production and destruction Circulating erythrocytes – the number remains constant and reflects a balance between RBC production and destruction –Too few red blood cells leads to tissue hypoxia –Too many red blood cells causes undesirable blood viscosity Erythropoiesis is hormonally controlled and depends on adequate supplies of iron, amino acids, and B vitamins Erythropoiesis is hormonally controlled and depends on adequate supplies of iron, amino acids, and B vitamins Regulation and Requirements for Erythropoiesis

27. Regulation of erythropoiesis

28. Respiratory pigments Hemoglobin Erythrocytes derive their colour from a complex protein called hemoglobin. This substance is composed of a pigment, heme, containing iron, and the protein glohin. Hemoglobin has the power to attract oxygen molecules and to hold them in a loose chemical combination known as oxyhemoglobin. It is said, therefore, to have a chemical affinity for oxygen.

29. HEMOGLOBIN

30. Respiratory pigments Myoglobin Hem is also part of the structure of myoglobin, an oxygen-binding pigment found in red (slow) muscles and in the respiratory enzyme cytochrome c. Porphyrins other than that found in hem play a role in the pathogenesis of a number of metabolic diseases (congenital and acquired porphyria, etc.) It may be the reserve pigments, which give the tissue oxygen in a small oxygen condition.

31. MYOGLOBIN

32. Methods for determination of hemoglobin To determine hemoglobin levels proposed many different methods: Gas metric method - measuring the amount of bound oxygen (1 g Hb can connect 1.36 ml of oxygen); Gas metric method - measuring the amount of bound oxygen (1 g Hb can connect 1.36 ml of oxygen); Iron metric - measuring iron levels in the blood (iron content in Hb is 0.34%); Iron metric - measuring iron levels in the blood (iron content in Hb is 0.34%); Colorimetric - comparing the color of the solution to the color of the blood of the standard solution. Colorimetric - comparing the color of the solution to the color of the blood of the standard solution.

33. Types of hemolysis of erythrocytes Osmotic Osmotic Mechanical Mechanical Thermal Thermal Biological Biological Chemical Chemical

34. Osmotic resistance of erythrocytes The minimum osmotic resistance of erythrocytes observed in fresh blood 0,50-0,45% NaCl, maximum - to 0,34- 0,32% NaCl. The minimum osmotic resistance of erythrocytes observed in fresh blood 0,50-0,45% NaCl, maximum - to 0,34- 0,32% NaCl.

35. The viscosity of the blood It is the friction that occurs between layers of fluid moving at different speeds. As in the bloodstream at different rates move blood cells and plasma, the viscosity just depends on them. Determine the figure using viscometer. Normally, blood viscosity is about 5 (as the viscosity of water is 1). It is the friction that occurs between layers of fluid moving at different speeds. As in the bloodstream at different rates move blood cells and plasma, the viscosity just depends on them. Determine the figure using viscometer. Normally, blood viscosity is about 5 (as the viscosity of water is 1).

36. Hematocrit Volume ratio of blood cells called plasma and hematocrit. Volume ratio of blood cells called plasma and hematocrit. Normally it is men 0,40- 0,48 l / l (40-48%) in women - 0,36-0,42 l / l (36-42%). Normally it is men 0,40- 0,48 l / l (40-48%) in women - 0,36-0,42 l / l (36-42%).

37. Erythrocyte sedimentation rate (ESR) Density or specific gravity of erythrocytes (1,098) is higher than plasma (1.027) and therefore in vitro with blood clot devoid of ability, they slowly settle to the bottom. Erythrocyte sedimentation rate in healthy male is 2-10 mm per hour, and a woman - 2-15 mm per hour. In newborns, it is only 1.2 mm per hour. Intensive physical work leads to decreased ESR. Pregnant sedimentation rate can reach 45 mm per hour. Density or specific gravity of erythrocytes (1,098) is higher than plasma (1.027) and therefore in vitro with blood clot devoid of ability, they slowly settle to the bottom. Erythrocyte sedimentation rate in healthy male is 2-10 mm per hour, and a woman - 2-15 mm per hour. In newborns, it is only 1.2 mm per hour. Intensive physical work leads to decreased ESR. Pregnant sedimentation rate can reach 45 mm per hour.