1. BIO - LAB

2. Biological control Very successful in cane crop Natural enemies of sugarcane periodically reliesed in field females of trichrogramma trace the eggs of cane borers and lay eggs there Within 7 days the parasitizer completes its life cycle Adults of trichrogramma emerge from cane borers egg.

3. Work in BIO-LAB Sitotraga (stored grain pest) Trichogramma (Parasitizere of eggs) Coutisia Fly (attack on Larvae)

4. Sitrotraga Stored Grain pest Feeded on wheat 100 kg of Wheat is Daily used in lab. Eggs of Sitotraga are parsitized by Trichogramma females SitoTraga rearing in lab is actually for Trichogramma,.

5. Rearing Technique of Trichrogramma (cards) in Bio-Lab

6. 1.Sitotraga propagation  Growth medium for propagation of Sitotraga is Wheat  Collection of adults emerging from wheat  Adults are provided Fine Flour For Egg laying  Sieving of eggs.  Eggs of Sito are glued on 2*2.50 inchespaper card

7. Trichograma propagation Each card contain 1500-2000 eggs Cards containing eggs are parasitized by trichogramma after parasitization eggs become blacks. after day of parasitization cards are ready to be released in field

8. Card Re alising Technique Release depend on data collected by IPM officers in Field At a time 10 cards are released /acre 8*8 metre distance. Take to field in cooler. Avoid direct sunlight Early in morning or evening Staple underside of leaves adult will emerge in 3-4 days only Periodically releae in field…

9. Advantages Top, Root,stem Borers can be controlled successfly. Safe for Beneficial Insects Easy to apply

10. Coutisia fly. Sting the larvea of stem Borer Female lays egg there Produce its generation Only female adult can infect In Lab maize Stem Borer Larvae is used as a medium for propagation.