1. Alzheimer’s & the possible treatments effects of melatonin and resveratrol Katharine Frost

2. Need Segal & Wayne, 2009 Figure 1

3. Fundamentals Christensen, 2007 Figure 2

4. Model Organisms & Types of Alzheimer’s - Apoe4 gene Korschun, 2006 PET brain scans reveal plaque and tangle accumulation in patients with the APOE-4 gene, which increases risk of Alzheimer's Segal & wayne, 2009, Figure 4 Figure 3

5. Non hereditary Alzheimer’s (Korschun, 2006) treatments with anti oxidants

6. Resveratrol & Melatonin Proteasome function Patton, 2005 Melatonin levels and age Ward, 2009 Figure 5 Figure 6

7. Literature Review mM Philippe Marambaud, Resveratrol Promotes Clearance of Alzheimer's Disease Amyloid-Peptides, The Journal of Biochemistry Figure 7

8. Literature Review Srinivasan (2006) Melatonin in Alzheimer's Disease and other Neurodegenerative Disorders, Behavior and Brain Functions Journal

9. Purpose and Hypothesis Purpose: the purpose of this experiment is to find out if resveratrol or melatonin slow down the progression of Alzheimer's disease Hypothesis : Drosophila treated with resveratrol and drosophila treated with melatonin will show less loss of cognitive ability than flies not treated

10. Methodology

11. Budget plan budget plan Katharine Frost 5/25/09 vendoritem order #quantitydescriptionprice (single)price (total) carolina1731205drosophila vials7.537.5 carolina1731255vial caps3.9519.75 carolina1731225vial plugs4.321.5 carolina1732101medium7.25 carolina1731901vial labels21 iherb.comJRW-140611resveratrol13.97 iherb.comnow-032571melatonin77 twpinc.com002X002C0630W36T1copper mesh10.87 $138.84 total

12. Do-Ability uses protocols and materials that have all been handled and dealt with before in the lab Much of the materials needed for caring for the drosophila are already available Copper mesh, and resveratrol and melatonin would be needed

13. Bibliography Bahr, Marthias; Neuro-protection, Alzheimer's Disease; Neuro-protection; 2004; page 34 Behkne, John A; Human aging; The biology of aging; published 1978, pages 182- 185 Conn, Michael; Drosophila models of aging; Handbook of Models for Human Aging; published 2006; pages 253-255 Christensen, DD; Alzheimer 痴 Disease: Progress in the Development of Anti-amyloid Disease-Modifying Therapies; CNS spectrums; Vol 12; 2007; pages 113- 123 Crowther, Damien; Alzheimer's, Working with Drosophila; www.research-horizons.com; issue 1; October, 2006 Dead, Ward; Neuroendocrine theory of aging chapter 5; http://www.vrp.com/articles.aspx?ProdID=338; 2009http://www.vrp.com/articles.aspx?ProdID=338 Korschun, Holly; Scientists Discover Possible Link Between Oxidative Stress and Non-hereditary Degenerative Disease; http://www.medicalnewstoday.com/articles/42423.php; May 2, 2006 Marambaud, Philippe; Resveratrol Promotes Clearance of Alzheimer's Disease Amyloid-Peptides; The Journal of Biochemistry; Vol 10; November 11, 2005; Pages 316-326 Patton, Dominique; Resveratrol Tackles Alzheimer's Plaques; http://www.nutraingredients.com/Research/Resveratrol-tackles-Alzheimer-s-plaques-shows-lab- study; November 4, 2005 Shenk, D; Alzheimers disease; The Columbia Encyclopedia; Vol 6; 2008 Srinivasan, V; Melatonin in Alzheimer's Disease and other Neurodegenerative Disorders; Behavior Brain Functions; Volume 2; May 4, 2006; 2-15 Wayne, Melissa and Segal, Jeanne; Alzheimer's Disease Symmptoms and Stages; www.helpguide.org; 2009.