1. Learning how to use the microscope

2. (COPY NOTES) 1.There are 2 kinds of microscopes: one lensmagnifying A. Simple: one lens (magnifying glass) B. Compound (Complex): 2 or more lenses

3. Light Microscope

4. Parts of the Microscope Revolving Nosepiece 1. Revolving Nosepiece

5. Parts of the Microscope 2. Lens/Objective

6. Parts of the Microscope 3. Stage

7. Parts of the Microscope 4. Disc Diaphragm (controls amount of light)

8. Parts of the Microscope 5. Light source: Lamp or light

9. Parts of the Microscope 6. Base

10. Parts of the Microscope 7. Eyepiece

11. Parts of the Microscope 8. Arm

12. Parts of the Microscope 9. Stage Clips

13. Parts of the Microscope 10. Coarse Focus/ Adjustment

14. Parts of the Microscope 11. Fine Focus/ Adjustment

15. Calculating Magnification Multiply lens powers together!

16. Eyepiece10 XEyepiece: 10 X by itself Low Power: 4red 40 X large objects/scanningLow Power: 4X; red color; and a TOTAL magnification of 40 X mainly used for large objects/scanning Medium Power: 10 X, yellow 100 XMedium Power: 10 X, yellow color; TOTAL magnification of 100 X High Power: 40 X;blue 400 X; mainly used for smaller objects/detailsHigh Power: 40 X; blue color; and a TOTAL magnification of 400 X; mainly used for smaller objects/details

17. Parts of the Microscope 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Eyepiece Revolving Nosepiece Objective Lens Stage Clips Stage Diaphragm Lamp/Light Base Arm Coarse Focus Fine Focus

18. Microscope Skills

19. When you are ready to start: LOW 3.Make sure microscope is on LOW power! 4.Put stage all the way UP (you focus by slowly moving stage down) 5.Check the light setting (should be open)

20. Other Things to Consider: 6.Carrying the Microscope: Always use 2 hands!

21. Other Things to Consider: HIGH 7.Never use the COARSE FOCUS KNOB while on HIGH power.

22. 8. When putting the microscope away (at the end of class): LOW A. It should be on LOW power. DOWN B. The stage should be all the way DOWN (do this with the coarse focus knob!) OFF C. The light switch should be OFF

23. 9.TROUBLESHOOTING What if you can’t get your object into focus? A. Check that you have one of the lenses clicked into place B. Check that your disc diaphragm is allowing light to pass through (not closed)

24. TROUBLESHOOTING (CONTINUED) LENS PAPERC. Clean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!) D. GO BACK TO LOW POWER AND START OVER!

25. MAGNETICUse a MAGNETIC field to bend beams of light; used to see things at much higher level of detail 1,000,000Can magnify images up to 1,000,000 times!

26. There are 2 kinds: SURFACESSCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACES of objects

27. There are 2 kinds: THROUGHTRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGH an object.

28. Electron Microscope Images: http://www.pbrc.hawaii.edu/bemf/microangela/

29. Electron Microscopes

30. Microscopes by Brainpop