2. rh Lecture 3 Slides

3. Michaelis-Menten Equation V o = V max S K m + S again, a rectangular hyperbola Maud Menten rh

4. Michaelis-Menten Equation V o = V max S K m + S rh when there is no S, V 0 is also 0 as S gets big, V 0 approaches V max saturable

5. fig 6-11

6. box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

7. 1/V o = V max S K m + S rhM & M inverted Lineweaver-Burk Plot

8. box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

9. In class I show a lot of fancy uses of the LB plot, but you just need to know the basic double reciprocal plot!!!

10. box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

11. BIOENERGETICS or Free energy and life

12. part II intro-1

13. part II intro-3

14. part III intro-4

15. patterns in metabolism there are many…

16. THE STORAGE and USE of CHEMICAL ENERGY in CELLULAR METABOLISM

17.  G =  H - T  S Gibbs free energy enthalpy (heat) entropy (order) “delta gee”- The change in free energy

18. entropy from increased numbers

19. ? - 1 atm pressure - water at 55M - pH = 7 -from initial concentrations of 1M - temperature 25 o C

20. K’ eq is predicted by  G’ o … table 13-3

21. table 13-2 A 10-fold change in Keq for each 1.4 kcal/mole

22. Actual free energy for a reaction concentration ratio when substrates are high- negative when products are high- positive

23. Actual free energy for a reaction concentration ratio Henri-Louis Le Chatlier

24. adenosine triphosphate… ATP

25. The 20$ bill of the cell!!!

26. table 13-4

27. Hydrolysis of ATP fig 13-11

29. table 13-6

30. fig 13-9

31. [ATP] [ADP][P i ] Actual ATP hydrolysis energy standard free energy= -30.5 kJ/mole actual free energy= -51.8 kJ/mole in erythrocyte

32. pg 494 free energies are additive!

33. spontaneity determined by whole set of reactions fig 13-11

34. What is needed to lift this 3 kg dog into the air? Something heavier than 3 kg!!!

36. free energies are additive! 11kg 68kg riddle: how do you lift 11 kg? ans: lower a weight > 11kg! In metabolism, it is all about the coupling mechanism

37. spontaneity determined by whole set of reactions (fig 13-11 ed 4)

38. a mechanism of energy coupling fig 13-8

39. Much of metabolism is about LEAVING GROUPS!!! fig 13-8 C.R.H. Raetz

40. fig 13-10

41. adding a good leaving group to form a sigma bond… palmitoyl-CoA + H 2 Opalmitate + CoA-SH This reaction is spontaneous in this direction … so how do we make run the reaction backwards? Ans: couple it to the hydrolysis of ATP

42. making a good leaving group to form a sigma bond…

44. driving fancy chemistry by… making a good leaving group! box 13-2