1. RF Generator Power 1550W Argon Gas Flow Rates Plasma15 L / Min Carrier0.8 L / Min Makeup0.35 L / Min Collision Gas (He) 4 mL / Min Spray Chamber Temp.2 °C Omega Lens11.0 V Octopole bias-18 V Energy Discrimination4.0 V Replicates3 NebulizerMicromist Concentric Isotopes monitored 55 Mn, 56 Fe, 59 Co, 63 Cu, 66 Zn Supple 1.

2. Supple 2.

3. Supple 3. 0 2 4 6 8 10 12 Necropsy (weeks)

4. Control 6 mg/kg Kidney Liver Control 6 mg/kg Supple 4.

5. Supple 5. SampleSolutionDiameter (nm)Surface charge (mV) Al-D In Water112.2 ± 31.853.78 ± 0.73 In GA137.7 ± 40.239.55 ± 0.43 Al-R In Water107.5 ± 30.972.44 ± 7.25 In GA92.9 ± 26.338.40 ± 1.35

6. (A) (B) Supple 6. Drinking water (% of control, ml/mice b.w.) Diet (% of control, g/mice b.w.)

7. ** * Supple 7.

8. Data for review only : Experimental design for consumption of diet and drinking water (n =3) Cage No.1Cage No.2Cage No.3 Diet and drinking water

9. Data for review only : Experimental design using the blood

10. 100 μl Lysis 100 μl ICP/MS analysis (n=3) 100 μl Data for review only : Experimental design for ICP/MS