1. Bacterial Requirements
Temperature and Oxygen

2. Measurement of Microbial Growth
CFU
Direct
Viable Plate Counts
Serial Dilutions
Pour Plate
Spread Plate
Membrane Filtration
Counting Chamber
MPN (fecal, H20, food)
Indirect
Turbidity
Metabolic activity

3. Metabolic Classifications
Temperature
Oxygen requirements pH
Pressure
Radiation

4. Growth Curve Labeled Phases
Lag phase
Adaptive
Start metabolism
1-3 days
Log phase
Generation time doubles
Most metabolically active
Stationary phase
Growth = death
Death phase
Requirements decrease
Possible spore formation

5. Temperature Requirements

6. Temperature Experiment
4 TSA plates
Label
Temperature
4 different settings
Wheel spoke pattern
5 Bacteria
4 SAB broth/Durham tube
S. cerevisiae

7. Anaerobic Culture Methods

8. Oxygen Requirements Obligate Aerobes Microaerophiles
Aerotolerant anaerobes
Obligate Anaerobes
Facultative Anaerobes
Capnophiles

9. BHI deeps Brain/Heart infusion Melt Label Inoculate Ice Bath
Organism
Inoculate
Pipette or loop
Roll tube to distribute
Ice Bath
37°C

10. Fluid Thioglycollate Broth
Sodium thyioglycollate
Reduction (redox)
Pink if oxidized
Label tubes
Thio
Organism
Inoculate with loop
37°C

11. Gas Pack Jar: CO2 anaerobic System
TSA agar plate
4 quadrants
Label bacteria
Label O2
H2O added to envelope to
Produce H2 and CO2
NaHCO3, NaBH4
H2 + O2 => H2O

12. Aerobic / Anaerobic Lab Test Results
aerobe FA
aerotolerant
anaerobe

13. Physical Requirements: Temperature
Psychrophile
Unsaturated FA in cell membrane
Cold temperatures
Psychrotroph (facultative psychrophiles)
Refrigeration
Room temperature
Mesophile
Warm Blooded Animals
Thermophile/Hyper-
Saturated FA in cell membrane

14. Bacterial Examples: Temperature
psychrophile
psychrotroph
mold
Vibrio
mesophile
thermophile
Archaea
E coli

15. Oxygen Requirement Classification

16. BHI results

17. Thio Tubes

18. Bacterial Examples of O2 Groups
Obligate Aerobes
Pseudomonas
Microaerophiles
H. pylori
Aerotolerant aerobes
Streptococcus
Lactobacillus
Obligate Anaerobes
Clostridium
Facultative Anaerobes
E. coli
Stapylococcus
Capnophilic
Campylobacter
E coli
Staph

19. pH Groups Acidophile Neutrophile Akaliphile Bacillus acidocaldarius
Lactobacillus acidophilus
Neutrophile
E. coli
Staphylococcus aureus
Akaliphile
Streptococcus pneumoniae
Nitrobacter sp.

20. Questions?