1. (+)(+)

2. Team Members Undergraduate Students Aman Arya, Biology (Waterloo) Joseph Chan, Molecular Genetics (Toronto) Hannah Fong, Immunology / Chemistry (Toronto) Lutz Kummer, visiting Biology (Waterloo) Emanuel Nazareth, Biomedical Engineering (Toronto) Martina Spranger, visiting Biology (Waterloo) Max Wong, Biomedical Engineering (Toronto) Graduate Students Seema Nagaraj, IBBME and Dept. of Electrical Engineering (Toronto) Matt Scott, Dept. of Applied Mathematics (Waterloo) Cindy Ying Yin Yip, IBBME (Toronto) Technical Support Andrew Hessel, Lab Manager, Mak Lab, University Health Network Faculty Advisors Prof. Stephen Davies, IBBME and Dept. of Electrical Engineering (Toronto) Prof. Brian Ingalls, Dept. of Applied Mathematics (Waterloo)

3. Two Designs 3436384042 Temperature (°C) 32 Bacterial Etch-A-Sketch Cell See Us Temperature Sensor

4. Cell See Us Tuneable Temperature Sensor: Design

5. R0010 Rfp R0011 I12007 Gfp Temperature Sensitive Dimerization LacI CI

6. R0010 Rfp R0011 I12007 Gfp Temperature Sensitive Dimerization LacI CI

7. R0010R0011 I12007 Temperature Sensitive Dimerization

8. R0010R0011 I12007 Temperature Sensitive Dimerization

9. R0010R0011 I12007 Temperature Sensitive Dimerization

10. Cell See Us Tuneable Temperature Sensor

11. T 1 T 2 T 3

12. Cell See Us Tuneable Temperature Sensor T 1 T 2 T 3

13. Cell See Us Tuneable Temperature Sensor T 1 T 2 T 3

14. Cell See Us Tuneable Temperature Sensor T 1 T 2 T 3 Transcription rate at T 1 Transcription rate at T 2

15. 3436384042 Temperature (°C) 32 lacI fp 1 fp 2 CI Increase in temperature decreases the repression by lacI Modeling Results

16. Bacterial Etch-A-Sketch: Design

17. Bacterial Etch-A-Sketch Write/Draw with Pipette filled with Lactose Design will glow green

18. Bacterial Etch-A-Sketch Erase using pipette filled with Tetracyclin Picture will cease to glow green

19. Bacterial Etch-A-Sketch

20. R0011 Gfp R0040 Lactose Binds with LacI Inactive Repressor TetR LacZ (Metabolizes Lactose) LacI Lactose

21. R0011R0040 TetR LacZ (Metabolizes Lactose) Lactose Binds with LacI Inactive Repressor Lactose

22. R0011R0040 TetR LacZ (Metabolizes Lactose) Tetracyclin Binds with TetR Inactive Repressor aTc

23. R0011R0040 LacZ (Metabolizes Lactose) Tetracyclin Binds with TetR Inactive Repressor aTc

24. R0011R0040 LacZ (Metabolizes Lactose) LacZ metabolizes Lactose

25. R0011R0040 LacZ (Metabolizes Lactose) LacZ metabolizes Lactose

26. R0011 Gfp R0040 TetR LacZ (Metabolizes Lactose) LacI LacZ metabolizes Lactose

27. Project Plan and Execution

28. Initial Plan May – June –Brainstorm and finalize design July – August –Obtain constructs and test September – October –Make revisions, fine tune model parameters, obtain and test new constructs

29. Actual Execution May – August –Brainstorm designs September –Found out we needed to prepare constructs ourselves –Reconfigure designs to use available parts –Recruitment of volunteers with wet lab experience October – Early Nov. –Undergraduates began assembly –Coincided with peak of undergrad midterm season

31. Parts Made BBa_J11042BBa_J11051 B0031+ C0056R0040+ E0433 BBa_J11052 R0011+ E0422 And possibly:

32. Lessons for Next Year Larger team Schedule undergraduate lab time more effectively Prepare team for assembly

33. Questions?