1. Solution Switch for Patch Clamp
Dan Carlson
Dave Ugai
Erik Bieging
Clients: Jinling Wang, Ph. D.
Gail A. Robertson, Ph.D.
Advisor: Professor John G. Webster

3. Problem Statement
Current method is a tedious process and an inefficient use of time
Our goal is to develop an automated system for delivering solutions to a patch clamp

4. Patch Clamp Background
Method used to study single cells
HERG potassium channel
Long QT syndrome

5. Current Equipment

6. Client Requirements
Must reliably open and close valves on command from the computer
Compatible with up to eight feeds of medical tubing
Must generate minimal noise
Compatible with pClamp-8 software

7. Variable Design Components
Valves: solenoid or solenoid pinch valve (both normally closed)
Valve Controller: Valvelink8, Controlled 8 relay driver, Manually assembled controller

8. Solenoid Valves

9. Solenoid Pinch Valves

10. Design Setup

11. Design Alternatives Valvelink8 controller with solenoid valves
Controlled 8 relay driver with solenoid valves
Manually assembled controller with solenoid valves

12. Design Matrix Valvelink8 8 relay driver Manually assembled Price 1 5 9
Simplicity 10 8 4
Reliability 7
Total 21 24 20

13. Chosen Design 8 relay driver
1/8 inch solenoid valves (normally closed)
12 volt switcher power supply, and serial adapter

14. Future Work Discuss design with client Order parts Assemble design
Configure with computer
Begin testing
Begin work on temperature controller

15. Questions?