1. Hematopoietic and Lymphatic System

2. Blood Parasites

3. Trypanosoma Trypanosoma cruzi Causing chagase disease. Trypanosoma bruci African sleeping disease.

4. morphological forms of Hemoflagellate

5. Trypanosoma gambianse brucii Vector Tse Tse fly. Trypomastigote stage in the blood stream. African sleeping disease.

6. Life Cycle of Trypanosoma brucei spp Life Cycle of Trypanosoma brucei sppLife Cycle of Trypanosoma brucei spp

8. Trypanosoma cruzi Vector: reduviid bugs. Disease: American Chagas disease. Trepomastigote stage in blood.

9. "pseudocysts" of Trypanosoma cruzi containing the amastigote stage in heart muscle

10. Life Cycle:

11. Plasmodium spp. P. falciparum. P. ovale. P. vivax. P.malariae

13. Plasmodium Falciparum a.Multiple ring stage inside RBC.

14. Plasmodium Falciparum Cresent or banana shape gametocyte

15. Plasmodium spp. Gametocytes of P. ovale.P. vivax. P.malariae. Round in shape Male -->Micro gamete --> diffuse. Female --> macro gamete --> condensed.

16. Plasmodium life cycle

17. Leishmaniasis Leishmaniasis is transmitted through the bite of female phlebotomine sandflies. Leishmania donovani (Visceral VL) Leishmania tropica (Cutaneous CL) Leishmania braziliensis ( Mucocutaneous leishmaniasis MCL)

18. Leishmania spp. Promastigotes stage in sand flies.

19. Amastigote in the tissue.

20. Leishmania life cycle

21. Bacteriology

22. Media and additives present in blood culture bottle Several media are used for blood culture bottles like. Trypticase soy broth. Thioglycolate media, Brain heart infusion.additives SPS (sodium poly anethel sulfonate)

23. Media used for Isolation Blood Media: most commonly used. Chocolate agar : Thioglycolate media: (enriched) MacConkey agar: Enterobacteria.

24. inoculation. And we do the culture by streaking method.

25. Swap method take the swap under sterile condition, rotate it on the first quadrant of blood agar plate and replace it in a thioglycolate broth.

26. Streptococcus pyogenes Colonies of Streptococcus pyogenes on sheep blood agar. Notice: * Presence of b hemolysis around colonies * Enhanced hemolysis around stabbing sites * Sensitivity to bacitracin (Disk A)

27. Streptococcus pyogenes Pin point colony : (white or gray) Gram stain: G+ve, cocci, single chain. Catalase enzyme: differentiate between Streptococcus –ve Staphylococcus +ve

28. Staphylococcus aureus G-positive cocci in clusters, typical of Staphylococcus aureus Catalase test used to differentiate Staphylococci from Streptococci

29. Coagulase test used to differentiate S. aureus from other Staphylococcus spp.

30. Mannitol salt agar: selective and differential for Staphylococcus spp. ( Staphylococcus aureus  manitol fermenter  yellow)

31. Pseudomonas aeruginosa Greenish discoloration of media due to production of pyocyanin by Pseudomonas aeruginosa

32. Pseudomonas aeruginosa Results of oxidase test

33. E. coli G-negative bacilli, typical of E. coli Colonies of E. coli on MacConkey agar (Pink color indicates lactose fermentation)

34. IMViC reaction of E. coli: + + - - KIA A/A

35. Proteus spp. Proteus - Members of the genus Proteus will swarm at certain intervals and produce a pattern of rings due to their motility.

36. Urease Test, H2S (Positive Urease Test)

37. THANK YOU