1. Different Kinds of Microscopes Learning how to use the microscope Click link below to watch video:

2. There are 2 kinds of microscopes: one lensmagnifying Simple: one lens (magnifying glass) Compound: 2 or more lenses

3. Watch this video clip: Simple vs. Compound Video Clip Simple vs. Compound Video Clip

4. Parts of the Microscope Revolving Nosepiece 1. Revolving Nosepiece

5. Parts of the Microscope 2. Lens/Objective

6. Parts of the Microscope 3. Stage

7. Parts of the Microscope 4. Disc Diaphragm (controls amount of light) 1 = lowest setting/ 1 = lowest setting/ 5 = highest setting

8. Parts of the Microscope 5. Light source

9. Parts of the Microscope 6. Base

10. Parts of the Microscope 7. Eyepiece

11. Parts of the Microscope 8. Arm

12. Parts of the Microscope 9. Stage Clips

13. Parts of the Microscope 10. Coarse Focus/ Adjustment (Big Knob)

14. Parts of the Microscope 11. Fine Focus/ Adjustment (Small Knob)

15. Calculating Magnification Multiply lens powers together!

16. Eyepiece10 XEyepiece: 10 X by itself Low Power: 4red 40 X large objects/scanningLow Power: 4X; red color; and a TOTAL magnification of 40 X mainly used for large objects/scanning Medium Power: 10 X, yellow 100 XMedium Power: 10 X, yellow color; TOTAL magnification of 100 X High Power: 40 X;blue 400 X; mainly used for smaller objects/detailsHigh Power: 40 X; blue color; and a TOTAL magnification of 400 X; mainly used for smaller objects/details

17. Other Things to Consider: Carrying the Microscope: Always use 2 hands!

18. Watch this Video Clip: Parts of the MicroscopeParts of the Microscope

19. When you are ready to start: LOW Make sure it is on LOW power! down The stage should be all the way down (you focus by slowly moving it up!) 1-2 to start Check the light setting (should be at 1-2 to start)

20. Other Things to Consider: HIGH Never use the COARSE FOCUS KNOB while on HIGH power.

21. TROUBLESHOOTING What if you can’t get your object into focus? Check that you have one of the lenses clicked into place IN BETWEENCheck that your disc diaphragm is in place (not IN BETWEEN settings)

22. TROUBLESHOOTING What if you can’t get your object into focus? LENS PAPERClean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!) GO BACK TO LOW POWER AND START OVER!

23. When putting the microscope away (at the end of the day): LOW It should be on LOW power. WRAPPED The cord should be WRAPPED around the base. DOWN The stage should be all the way DOWN (do this with the coarse focus knob!) OFF The light switch should be OFF

24. MAGNETICUse a MAGNETIC field to bend beams of light; used to see things at much higher level of detail 1,000,000Can magnify images up to 1,000,000 times!

25. There are 2 kinds: SURFACESSCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACES of objects

26. There are 2 kinds: THROUGHTRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGH an object.

27. Electron Microscope Images Go to this website and check out the cool images! Answer the 2 questions on your notes page. http://www.pbrc.hawaii.edu/bemf/microangela/