1. Comparison of Modified ETDRS and Mild Macular Grid Laser Photocoagulation Strategies for Diabetic Macular Edema Sponsored by the National Eye Institute, National Institutes of Health, U.S. Department of Health and Human Services

2. Objective  Compare two laser photocoagulation techniques:  Modified ETDRS focal photocoagulation (mETDRS) to areas of edema Direct treatment to microaneurysms Grid to diffuse leakage  Mild macular grid (MMG) laser technique small mild burns throughout macula in areas with and without edema no direct treatment of microaneurysms

3. Background  Presumed mechanism of focal photocoagulation include  Closure of microaneurysms  Reduced blood flow leading to auto-regulation and reduced edema  Improved oxygenation leading to auto-regulation and reduced edema  Stimulation of biochemical processes in RPE  Would light widespread laser (mild macular grid, MMG) to the macula be effective?  Pilot clinical trial

4. 4 Study Design mETDRS N=162 EyesMMG N=161 Randomized Clinical Trial (Pilot Study) Major Eligibility Criteria Assessed: >18 years old Type 1 or type 2 diabetes Study eye meets the following criteria (subjects allowed 2 study eyes):  Best corrected electronic ETDRS visual acuity score of ≥19  Definite retinal thickening on clinical exam due to previously untreated DME  Retinal thickness measured on OCT of 250 μm or more in the central subfield or 300 μm or more in at least 1 of the 4 inner subfields  Had no prior laser or other treatment for DME. Subjects with 2 study eyes: 1 eye was randomly assigned to receive 1 treatment and 1 eye was assigned to receive the other.

5. Mild Macular Grid 40 minutes post laser treatment 6 weeks post laser treatment

6. ...... Follow-up and Treatment Schedule 3.5 Month ± 2 Weeks 3.5 Month ± 2 Weeks 12 Month ± 4 Weeks 12 Month ± 4 Weeks...... 8 Month ± 4 Weeks 8 Month ± 4 Weeks Primary outcome: Change in OCT Secondary Outcome: Change in visual acuity (Method: Repeated measures least squares regression models)  Measurements by certified Evaluator Best-corrected electronic ETDRS visual acuity OCT-measured retinal thickness  Macular laser photocoagulation was repeated if DME persisted and such treatment was warranted in the opinion of the investigator, according to the treatment guidelines.

7. Baseline Demographics and Clinical Characteristics Subject Characteristics mETDRS Group (N=162 eyes) MMG Group (N=161 eyes) Gender: Women - N(%)38%43% Age (yrs) - Mean+SD58+1159+11 Race - N(%) White63%64% African-American18%19% Hispanic or Latino10%8% Asian5%4% Other4%

8. Baseline Demographics and Clinical Characteristics mETDRS Group (N=162 eyes) MMG Group (N=161 eyes) Diabetes Type - N(%) Type 17%6% Type 293%94% Duration of Diabetes (years) - Mean+SD 14+913+8 HbA1c (%) - Mean+SD8.2+1.98.2+2.1

9. Baseline Demographics and Clinical Characteristics Cont. Ocular Characteristics mETDRS Group (N=162 eyes) MMG Group (N=161 eyes) E-ETDRS Visual Acuity (letter score) - N(%) > 84: 20/20 or better27%20% 83-69: < 20/20 to 20/4047%57% 68-49: < 20/40 to 20/10022%17% 48-34: < 20/100 to 20/2004% 33-19: < 20/200-20/4000%2% Mean+SD - letters74+1273+14

10. OCT mETDRS Group (N=162 eyes) MMG Group (N=161 eyes) Central Subfield Thickness (microns) Mean+SD 335+128346+118 Maximum retinal thickening of central and inner subfields (microns, see text) Mean+SD 148+122163+111 Number of eyes with at least 1 unthickened subfield - N(%) 60%55% Baseline Demographics and Clinical Characteristics Cont.

11. Mean Central Subfield Thickness

12. Percent Edema Resolved for Central Subfield Thickness P=0.29 P=0.23 P=0.56

13. Percent of Edema Resolved for Weighted Inner Zone Thickness Note: Weighted inner zone thickness is a weighted average of the thickness in the central and 4 inner subfields P=0.57 P=0.02 P=0.07

14. Percent of Edema Resolved for Maximum Retinal Thickening Note: Maximum thickening is the maximum amount of excess thickness of the central and 4 inner subfields P=0.57 P=0.26 P=0.93

15. Percent of Edema Resolved for Retinal Volume Note: Retinal volume is a weighted average of the thickness in the central, 4 inner and 4 outer subfields converted to mm 3 P=0.31 P=0.01P=0.12

16. Percent with ≥ 10 Letter Improvement in Visual Acuity

17. Percent with ≥ 10 Letter Worsening in Visual Acuity

18. Summary  Maximum retinal thickening in the inner zone (within 1500 microns of macular center), central subfield thickness, weighted inner zone thickness and retinal volume decreased in both groups  MMG less effective than mETDRS in reducing retinal thickening  Visual acuity similar in both groups