1. Today: Inquiry 2 and Lab Safety... http://www.eurofins.com/media/19528/es_breda_lab_overview.jpg

2. Why use a sample instead of a population? Logistics Cost Time

3. Samples: Random- each member of population has an equal chance of being part of the sample. or Representative- ensuring that certain parameters of your sample match the population.

4. Replicates: Technical vs Experimental Technical replicate- one treatment is divided into multiple samples. Experimental replicate- different, replicate, treatments are done to different samples.

5. Testing blood sugar levels after eating a Snickers:

6. Divide a participants blood into 3 samples and test blood sugar in each sample. Technical or Experimental replicate?

7. Testing blood sugar levels after eating a Snickers: Test 3 different people. Technical or Experimental replicate?

8. Testing blood sugar levels after eating a Snickers: Test the same person on 3 different days. Technical or Experimental replicate?

9. What sample size do you need?

10. It depends on the error you expect.

11. To determine an appropriate sample size, you need to estimate a few parameters. Means Standard Deviation Power: The probability that an experiment will have a significant (positive) result, that is have a p-value of less than the specified significance level (usually 5%).

12. This calculator will help you determine the appropriate sample size: http://www.stat.ubc.ca/~rollin/stats/ssize/n2.html

13. What sample size do you need? It depends on the error you expect. (So it is impossible to predict with 100% accuracy before the experiment is carried out.)

14. Inquiry 2 -Who? Groups of 3-5 people

15. Inquiry 2 -Who? Groups of 3-5 people Divide work

16. Inquiry 2 -Who? Groups of 3-5 people Divide work Be clear about responsibilities and expectations Leave enough time to get everything done without panic

17. Inquiry 2 -What? Incredibly, amazingly, fantastically, critically important, essential information has been posted on the class webpage. Lab Safety Supplies Order Forms Updated Proposal and Report Formats

18. Inquiry 2 -What? May use gadgets, chemicals, kits, etc -https://uteach.utexas.edu/direct -Analytical/physical chemistry storeroom (order form) -Media kitchen (via chem storeroom) -Research Streams

20. CAS registry numbers: unique identifiers for most chemicals http://www.cas.org/expertise/cascontent/registry/regsys.html

22. Inquiry 2 -Where and When? You can work in PAI 4.14 during your scheduled lab times, and/or during my other lab times. M 12-3pm M 3-6pm Th 12-3pm Th 3:30-6pm

23. Inquiry 2 -Where and When? You can work during the your scheduled lab times, and/or during my other lab times. Outside of lab times, you may only work when a TA is present. These times will be posted weekly.

24. Inquiry 2 -Where? Lab Safety

25. Inquiry 2 -Where? Lab Safety Closed toed shoes, sleeves, legs covered to knees, hair out of harms way

26. Inquiry 2 -Where? Lab Safety Closed toed shoes, sleeves, legs covered to knees, hair out of harms way NO FOOD in lab

27. Inquiry 2 -Where? Lab Safety Closed toed shoes, sleeves, legs covered to knees, hair out of harms way NO FOOD in lab Be aware of what you are doing ans working with

28. Inquiry 2 -Where? Lab Safety Closed toed shoes, sleeves, legs covered to knees, hair out of harms way NO FOOD in lab Be aware of what you are doing ans working with Wash hands before leave lab

29. Inquiry 2 -Where? Waste Disposal You are responsible for the safe disposal of all waste you generate.

30. Inquiry 2 -Where? Waste Disposal

31. Inquiry 2 -Where? Waste Disposal You are responsible for the safe disposal of all waste you generate. This includes chemical waste and biological waste.

32. If you make a mistake, spill something, have an accident… tell someone immediately. http://www.time4science.com/images/labsafety/labsafety2.JPG

33. Inquiry 2 -How? Proposal Mostly the same as inquiry 1 2 additional parts

34. Inquiry 2 -How? Proposal Mostly the same as inquiry 1 2 additional parts In the Experiment section Include detailed information on equipment and reagents to be used. Where will you get them Needed order forms

35. Inquiry 2 -How? Proposal Mostly the same as inquiry 1 2 additional parts Add a Responsibilities section Describing who will do what…

36. Inquiry 2 -How? Proposal Proposals will not be approved until sources for all equipment and/or chemicals are secured. Orders will be placed during lab, after your proposal is approved.

37. Inquiry 2 -How? Written Reports Similar to inquiry 1, but add a Responsibilities section

38. Inquiry 2 -How? Written Reports Similar to inquiry 1, but add a Responsibilities section Everyone should participate in writing, some may write the original text, while others work on figures/tables, and others edit.

39. Inquiry 2 -How? Oral Reports Should be 15 to 20 minutes long

40. Inquiry 2 -How? Oral Reports Should be 15 to 20 minutes long Include all of the basic information from the written report. (Do not simply read your written report)

41. Inquiry 2 -How? Oral Reports Should be 15 to 20 minutes long Include all of the basic information from the written report. (Do not simply read your written report) Everyone will present some part of the oral report

42. Inquiry 2 -How? Oral Reports Should be 15 to 20 minutes long Include all of the basic information from the written report. (Do not simply read your written report) Everyone will present some part of the oral report Should be visually engaging

43. My group sucks, and now I am going to fail.

44. Don’t have a cow man. Your will be graded as a group and also only on your sections.

45. Inquiry 2 proposals are due in lab on Th 10/1 or M 10/5 Homework #4 due now: What was the hardest part of inquiry 1, and how will you make this easier for inquiry 2? This weekend: Pecan Street Arts Festival (http://www.oldpecanstreetfestival.com) Next week: Research Ethics