1. 2 Component Signaling 20.20(S11) N Kuldell

2. As simple as the box it comes in… http://www.dropclicked.com/practestian1anssheet_files/slide0022_image001.jpg Stimulus carbon source pH oxidative stress temperature bacterial density nitrogen osmotic stress …

3. As simple as the box it comes in… Stimulus carbon source pH oxidative stress temperature bacterial density nitrogen osmotic stress …

4. As simple as the box it comes in… StimulusResponse carbon sourcemotility, chemotaxis pHstress, virulence oxidative stresssuperoxide dismutase temperatureheat shock proteins syn bacterial densityquorum response nitrogenmetabolic changes osmotic stressuptake/output changes …

5. As simple as the box it comes in… StimulusResponse2CS carbon sourcemotility, chemotaxisCheA/CheY pHstress, virulencePhoP/PhoQ oxidative stresssuperoxide dismutase temperatureheat shock protein syn bacterial densityquorum responseLuxN,Q/LuxO nitrogenmetabolic changesNRI/NRII osmotic stressuptake/output changesEnvZ/OmpR … “Two Component Signaling (2CS)” for many --- not all---responses

6. The 2CS paradigm: in general Sensor protein Response regulator

7. The 2CS paradigm: Phosphorelay rxns Slide from Mike Laub

8. Sensor P ATP ADP External stimulus or ligand Sensor RXN 1: Autophosphorylation in response to stimuli The 2CS paradigm: Phosphorelay rxns

9. RXN 1: Autophosphorylation in response to stimuli The 2CS paradigm: Phosphorelay rxns RXN 2: Phosphotransferase from Sensor to Response regulator Sensor P Responder (inactive) Responder P Sensor

10. The 2CS paradigm: Phosphorelay rxns RXN 2: Phosphotransferase from Sensor to Response regulator RXN 3: Phosphatase inactivates Response regulator, resets system P Sensor Responder (inactive) Sensor Responder P Sensor RXN 1: Autophosphorylation in response to stimuli

11. 2CS examples: Osmoregulation http://www.plantphysiol.org/cgi/content-nw/full/117/3/723/F1

12. 2CS examples: Osmoregulation 2CS transduction, Hoch and Silhavy

13. SprE OmpR EnvZ PhoB PhoR CusR CusS DcuR DcuS NarL NarX NarP NarQ KdpE KdpD QseB QseC YedW YedV YfJR YfhK YehT YehU RcsB RcsC OmpR ArcB RstA RstB UhpC UhpB TorR TorS BasR BasS PhoP PhoQ CpxR CpxA CheY CheA AtoC AtoS UvrY BarA HydG HydD BaeR BaeS CheB RcsD NtrC NtrB FimZ CreB CreC DpiB DpiA animation: Mark Gouilan slide from Mike Laub YpdB YpdA EvgA EvgS what prevents unwanted cross-talk and ensures specificity? can we rationally rewire pathways? how do new pathways get integrated? Coordinating multiple signaling systems

14. As simple as the box it comes in… Light Sensing Device Color Generating Device PhotonsColor 1 (= cells are in the light)0 0 (= cells are in the dark)1

15. As simple as the box it comes in… Light Sensing Device Color Generating Device

16. the end