1. 1 10 mL of TSB Source Vial of TSB 20 or 30 mL shielded evac vial (simulated Tc-99m) X 4 Simulated Kit vial #1 1 mL 3 mL (repeat 3 more times for a total of 4 vials) 3 mL Shielded syringe 10 mL unshielded syringe Draw 0.4 mL of media from Kit Media Fill Procedure (simulated normal saline)

2. 2 3 mL shielded syringes 10 or 20 mL incubation vials Draw 0.4 mL of media from Kit Media Fill Procedure (Cont’d) Kit # 1Kit #2Kit #3Kit #4 Repeat 3 times for a total of 4 vials and 24 syringes

3. 3 Media Fill Procedure (Cont’d) Incubate 4 new vials at 25-35 Degrees Celsius in a controlled environment for 14 days. 2341

4. 10 mL of TSB Vial of TSB 20 or 30 mL shielded evac vial Tc-99m X 4 Simulated Kit vial #1 1 mL 3 mL (repeat 4 times) 5 mL Shielded syringe 10 mL unshielded syringe 3 or 5 mL shielded syringes Draw 0.4 mL of media from Kit Incubate vials at 25-35 Degrees Celsius Media Fill Procedure (Overview) 10 or 20 mL incubation vials 1 Repeat process 4 times for a total of 24 incubation vials