1. Microscopes

2. Development of the microscope Anton van Leeuwenhoek Anton van Leeuwenhoek Father of Microbiology Father of Microbiology Made his own lenses (some as powerful as the microscopes we use in class) Made his own lenses (some as powerful as the microscopes we use in class) First person to see living microorganisms First person to see living microorganisms

3. Type of microscopeFocuses what Through /Surface Magnifies up to Live organisms? Microscopes

4. Compound light microscope Focuses light Focuses light Shines light through objects Shines light through objects Magnifies up to 1000 times (ours are 400x) Magnifies up to 1000 times (ours are 400x) Can view live organisms Can view live organisms Uses combination of lenses Uses combination of lenses www.science.smith.edu/.../Labmanual/01CELLS.htm

5. http://www.bioweb.uncc.edu/1110Lab/notes/notes1/labpics/Elodea%20Leaf40c.jpg

6. Dissecting microscope Focuses light Focuses light See surface of objects See surface of objects Magnifies up to 40 times Magnifies up to 40 times Can view live organisms Can view live organisms Uses combination of lenses Uses combination of lenses wappingersschools.org/.../visualvocab/page4.html

7. http://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/charophyceans/Chara_d2_LApond2e_3.15x.jpg

8. Electron microscope Two types: Transmission electron microscope (TEM) Transmission electron microscope (TEM) Scanning electron microscope (SEM) Scanning electron microscope (SEM)

9. Transmission electron microscope Focuses electrons Focuses electrons Sees through objects Sees through objects Magnifies up to 100,000 times Magnifies up to 100,000 times Can view only dead or inanimate objects Can view only dead or inanimate objects www.phy.cuhk.edu.hk/.../CM120/CM120.html

10. Transmission electron microscope Mammalian mitochondrion biology.unm.edu/.../Summaries/Cell.html

11. Scanning electron microscope Uses electrons Uses electrons View surface of objects View surface of objects Magnifies up to 10,000 times Magnifies up to 10,000 times Views only dead or inanimate objects Views only dead or inanimate objectshttp://gsc.nrcan.gc.ca/ebeam/sem_e.php

12. Scanning electron microscope Bee eye www.rdg.ac.uk/cfam/facilities/sem.htm Bee eye www.rdg.ac.uk/cfam/facilities/sem.htm

13. Viruses budding from a cell from a cell Transmission electron microscope Scanning electron miscroscope www. aecom.yu.edu/aif/gallery/sem/sem.htm

14. JEOL microscope Uses finely-focused electrons Uses finely-focused electrons surface surface Magnifies up to at least 2,000,000 times Magnifies up to at least 2,000,000 times Cannot view live organisms Cannot view live organisms