1. Loschmidt Laboratories Enantis, Ltd. PROTEIN ENGINEERING

2. LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team

3. LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno

4. LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology

5. LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology  bioinformatics and molecular modelling

6.  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology  bioinformatics and molecular modelling  biochemistry and biophysics LOSCHMIDT LABORATORIES & ENANTIS

7. BIOSENSING DECONTAMINATION BIOCATALYSIS

8. DEVELOPMENT OF BIOCATALYTIC PROCESS

9.  GENETIC DATABASES  EXTREMOPHILES DEVELOPMENT OF BIOCATALYTIC PROCESS

10.  DIRECTED EVOLUTION resistance to organic solvents DEVELOPMENT OF BIOCATALYTIC PROCESS

11.  DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement DEVELOPMENT OF BIOCATALYTIC PROCESS

12.  DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement DEVELOPMENT OF BIOCATALYTIC PROCESS

13.  DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement 20°C DEVELOPMENT OF BIOCATALYTIC PROCESS

14.  OPTIMIZED EXPRESSION  FERMENTATION  DOWN STREAM DEVELOPMENT OF BIOCATALYTIC PROCESS

15.  MEDIUM ENGINEERING  IMMOBILISATION DEVELOPMENT OF BIOCATALYTIC PROCESS

16. HOTSPOT WIZARD Hot Spot Wizard RCSB PDB user

17. Hot Spot Wizard RCSB PDB CSA UniProt user PDBSWS HOTSPOT WIZARD

18. Hot Spot Wizard RCSB PDB CSA UniProt user CAVER CASTp PDBSWS HOTSPOT WIZARD

19. Hot Spot Wizard RCSB PDB CSA UniProt user CAVER CASTp PDBSWS tunnels Cd-hit BLASTNRDB MUSCLE Rate4Site HOTSPOT WIZARD

20. low high mutability HOTSPOT WIZARD

21. 1,3-1,4-  -glucanase PDB-ID 1GBG haloalkane dehalogenase PDB-ID 1BN6 phosphotriesterase PDB-ID 1I0D  -lactamase PDB-ID 1BLS HOTSPOT WIZARD

22. Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD positive and neutral

23. Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD positive and neutral slightly negative

24. positive and neutral slightly negative deleterious Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD

25. Pavelka et al. Nucl. Acids Res. 37: W376 2009