1. Side effect of transgenic plants Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová Biology Centre, ASCR, v.v.i. Institute of Entomology Department of Experimental Ecology Oxana Habuštová

2. Conducted research Monitoring of selected insect taxa and spiders on ten 0.5-ha plots in a 14-ha field in 2002-2005. Maize MON810 expressing Cry1Ab was compared with a parental, non-transgenic cultivar. Ten plants and five pitfall traps per plot were examined in 2-week intervals.

3. Field trials

4. Average temperatures

5. Precipitation profile

6. Quantification of Cry toxin

7. Content of Cry1Ab/c (µg/g) in MON810 in 2003-2005 (no toxin was detected in the control maize)

8. Numbers of dominating insects per ten plants in 2003-2005

9. Abundance of sucking insects in relation to Bt expression, date and year

10. Dominant carabid species

11. Dominant spider species

12. Abundance of epigeic arthropods on Cry1Ab (Bt), data, year, and plot position

13. Maize MON810 contains > 1 ppm Cry1Ab in the stem and folliage but undetectable amounts in the flowers and grain. It is not attacked by the European corn borer. None of the other herbivores living on the maize plants and none of their predators were affected. The compositions of epigeic beetles and spiders was not altered due to Cry presence. Conclusions

14. Recent experiment Will performed in the corner of 60ha field 14 ha will divided into 25 plots of 0.5ha each MON 88017, isogenic, treated with insecticides, reference cultivar A and B will be sowed Weather condition Precipitation profile Plant dwelling insect Soil insects by pitfall traps Entomopathogenic nematodes Yellow sticky traps with pheromone „Csalomon PAL“ for Diabrotica virgifera Yellow sticky traps for natural enemies Content of Cry3Bb1 toxin

15. Design of experimental field in 2009-2011

16. Thank you for your attention!