1. Pituitary Gland Dr. Hany Ahmed Assistant Professor of Physiology (MD, PhD). Al Maarefa Colleges (KSA) & Zagazig University (ARE) Specialist of Diabetes, Metabolism and Obesity Zagazig Obesity Management & Research Unit Dr. Hany Ahmed Assistant Professor of Physiology (MD, PhD). Al Maarefa Colleges (KSA) & Zagazig University (ARE) Specialist of Diabetes, Metabolism and Obesity Zagazig Obesity Management & Research Unit

2. Lecture Objectives Explain hypothalamus as the major integrative site for the neuroendocrine system. Contrast anterior and posterior pituitary lobes with respect to cell types, vascular supply, development, and innervations. Identify posterior pituitary hormones (oxytocin & vasopressin). List target tissues for oxytocin and describe its effects on each. Describe stimuli and mech. that control vasopressin secretion. Describe the general characteristics of hypothalamic releasing and inhibiting hormones and describe their route of transport from the hypothalamus to the anterior pituitary. Identify appropriate hypothalamic hormones that control the secretion of each of the anterior pituitary hormones. Diagram short-loop and long-loop negative feedback control of anterior pituitary hormone secretion.

3. The pituitary is two fused glands

5. Pituitary Gland (Hypophysis) Pea sized mass of glandular tissue Lies in sella turcica Slender stalk: Infundibulum connects pituitary gland to hypothalamus 2 parts: –Posterior pituitary (=neurohypophysis) Composed of nervous tissue –Anterior pituitary (=adenohypophysis) Consists of glandular epithelial tissue

6. Pituitary Gland Secretions Anterior Pituitary: secretes 1)Growth Hormone (GH) 2)Prolactin (PRL) 3)Thyroid Stimulating Hormone (TSH) 4)AdrenoCorticoTropic Hormone (ACTH) 5)Follicle Stimulating Hormone (FSH) 6)Luteinizing Hormone (LH) Posterior Pituitary: secretes 1)Vasopressin (ADH) 2)Oxytocin

9. I. Posterior Pituitary (=Neurohypophysis)  Along with hypothalamus forms neuroendocrine system.  Does not actually produce any hormones.  ADH & Oxytocin are formed mainly in supraoptic and paraventricular nuclei in hypothalamus respectively.  Transported as granules by Hypothalamo-Hypophyseal Nervous Tract.  Stores & releases two small peptide hormones (9 A.A.): 1- Vasopressin (ADH): conserves water during urine formation. 2- Oxytocin: stimulates uterine contraction during child- birth and milk ejection during breast-feeding.

10. Relationship of Hypothalamus & Posterior Pituitary (Hypothalamo-Hypophyseal Nervous Tract) 1.Vasopressin & oxytocin are synthesized in supraoptic & paraventricular nuclei in the hypothalamus. 2.The hormone travels down the axon to be stored in the neuronal terminals within the posterior pituitary. 3.On excitation of the neuron, the stored hormone is released from these terminals into the systemic blood for distribution throughout the body.

12. 1- ADH (=Vasopressin)  Vasopressin receptors: V1 receptors in blood vessels, and V2 in kidney tubules.  Functions: 1- Anti diuresis (=  reabsorption of water by kidneys): ADH   permeability of distal tubules & collecting ducts to water   water reabsorption by passive diffusion. Mechanism:  cAMP in cells & Formation of microtubules in cell membrane (Aquaporin 2). 2- Vasoconstrictor effect: in large dose. 10% decrease in blood volume is sufficient to cause release of ADH.

13. MECHANISM OF ACTION OF ADH

14. Regulation of ADH 1)  Osmolarity of ECF increses ADH release. 2)  Blood volume as in hemorrhage   frequency of inhibitory impulses from volume receptors to supraoptic nuclei   release of ADH. 3)Renal ischemia due to hemorrhage   Renin   Angiotensin II   ADH secretion   water reabsorption. 4)Alcohol  inhibits ADH secretion  marked diuresis. 5)Anxiety, Pain, Trauma, Morphine, Nicotine & Hot Weather   ADH.

16. Diabetes Insipidus (DI)  ADH secretion or action. Causes: 1)Central DI: Damage of supraoptic -hypophyseal system by a tumor. 2)Nephrogenic DI: Lack of kidney response to ADH (V2 receptor defect). Manifestations: Polyuria (4-6 L/day & may be 15 L/day)  loss of fluid in urine  dehydration  Polydipsia (constant thirst due to dehydration). Treatment: Synthetic vasopressin (= desmopressin) nasal spray.

18. Syndrome of Inappropriate ADH Secretion (SIADH) Causes: 1-  ADH from brain tumors & lung tumors. 2-  ADH from posterior pituitary in T.B. & pneumonia. Manifestations:  ECF volume (= water intoxication),  Na + concentration & osmolality (= dilutional hyponatraemia)  intracellular edema  convulsions & coma  sudden death. Treatment: 1- Loop diuretics with saline. 2- Vasopressin - 2 receptor antagonists. 3- Water restriction. 4- Demeclocycline.

20. 2- OXYTOCIN ACTIONS: On uterus: Stimulates pregnant uterus during Labor  Powerful contraction and helps delivery. Primary fertilization of ovum: Sexual Stimulation during intercourse  stimulation of paraventricular nuclei   oxytocin   uterine contractions  uterine suction of semen upward toward Fallopian tubes. Milk Ejection: Oxytocin  contraction of myoepithelial cells around alveoli of Mammary glands during Lactation.

21. OXYTOCIN

22. II. Anterior pituitary (=Adenohypophysis)  Cells: 1)Chromophobes: (50% of cells) = precursor cells. 2)Acidophils (alpha cells): (40% of cells) & secrete: Growth hormone (GH) & Prolactin (PRL). 3)Basophils (Beta cells): (10% of cells) & secrete: Thyriod stimulating hormone (TSH) or Thyrotropin. Adrenocorticotrophic H. (ACTH) or Corticotropin. Follicle stimulating hormone (FSH). Luteinizing hormone (LH).

23. Hypothalamo - Hypophyseal portal system Vascular connection between hypothalamus & anterior pituitary. Hypothalamus secretes polypeptide hormones (= hypothalamic releasing or inhibiting factors) absorbed into hypothalamic- hypophyseal portal capillaries to be carried to anterior pituitary.

24. Hypothalamic - hypophyseal Portal system

25. Vascular Link Between the Hypothalamus and Anterior Pituitary

26. Hypothalamic Releasing and Inhibiting Hormones

28. Anterior Pituitary Hormones

29. Anterior pituitary hormones

30. 1)Hypothalamus 2)Pituitary stimulation from hypothalamic trophic hormones 3)Endocrine gland stimulation from pituitary trophic hormones Endocrine Control Three Levels of Integration

31. Hormones of hypothalamic-anterior pituitary pathway

32. Negative Feedback Loops Regulate amount of hormones in blood Hormone levels stay within range needed for appropriate responses Eliminates waste of hormones. A) long loop –ve feedback: 1) Direct -ve feedback Anterior pituitary hormones  stimulate target gland (  TSH   Thyroxin). E.g. ↓ Thyroxin  ↑ TSH from Ant. pituitary ↑ Thyroxin  ↓ TSH.

33. 2) Indirect -ve feedback When hypothalamus is indirectly involved in regulation of hormone secretion from target gland via releasing or inhibitory factors {TRF   TSH   Thyroxin}. E.g. ↑ Thyroxin  ↓ TRF. ↓ Thyroxin  ↑ TRF. B) Short loop -ve Feedback: Some anterior pituitary hormones e.g. Somatotropin (= GH) can regulate its own release by a short loop -ve feedback on the secretion of its hypothalamic releasing or inhibitory factors. E.g. Somatotropin and Somatotropin RF or Somatostatin.

34. Negative Feedback Loops

37. Chapter 18 The Central Endocrine Glands Human Physiology by Lauralee Sherwood ©2010 Brooks/Cole, Cengage Learning