2.  What is it?

3.  What are they?

4.  What is it?  How does it work?  DNA is isolated  DNA is copied with PCR  Cut with restriction enzymes  Run through charged gel box  Compared

6.  What are they?  How are they made?  PCR makes lots of copies of the gene  Chosen gene is cut with restriction enzymes, leaving “sticky ends”  Circular bacterial DNA is cut with same restriction enzymes, leaving “sticky ends”  Cut pieces are joined together, so bacteria now produces protein in gene