1. Bacteria Biology 342

2. Prokaryotes and Eukaryotes Prokaryotes – single celled organisms lacking a membrane bound nucleus or organelles. All their DNA and proteins are together in the cytoplasm enclosed by the cell membrane. Prokaryotes – single celled organisms lacking a membrane bound nucleus or organelles. All their DNA and proteins are together in the cytoplasm enclosed by the cell membrane. Eukaryotes – some single celled, but mostly multicellular organisms with DNA enclosed in a membrane – the nucleus, and having organelles such as chloroplasts, mitochondria, Golgi bodies. Eukaryotes – some single celled, but mostly multicellular organisms with DNA enclosed in a membrane – the nucleus, and having organelles such as chloroplasts, mitochondria, Golgi bodies.

3. Interesting facts Two prokaryote Domains: Archaea and Bacteria Two prokaryote Domains: Archaea and Bacteria Prokaryotes are extremely promiscuous with their DNA – sequences are exchanged among different species and even different Kingdoms Prokaryotes are extremely promiscuous with their DNA – sequences are exchanged among different species and even different Kingdoms Species concept (BSC) breaks down with bacteria Species concept (BSC) breaks down with bacteria Bacteria diversity is more like genetic clusters or groups which are often termed genospecies Bacteria diversity is more like genetic clusters or groups which are often termed genospecies

4. Archaea (Archaebacteria) and Bacteria (Eubacteria) Archaea Cell wall: single compound Spores: absent – no dormancy Habitat: extremely harsh environments such as hot springs, salt lakes, oceans, and within the guts of ruminants and including humans Few or no pathogens Bacteria Cell wall: multiple compounds Spores: present – can remain dormant for years Habitat: ubiquitous – found everyplace on earth from hot springs, lakes, streams, oceans, soil, within plants and animals A large variety of pathogenic types

5. Prokaryote Cell

6. Size

7. Bacteria Classification The Gram stain, a crystal violet dye separates two groups of bacteria based on structural differences of their cell wall. The Gram stain, a crystal violet dye separates two groups of bacteria based on structural differences of their cell wall. Gram positive bacteria retain the dye color and are violet or purple Gram positive bacteria retain the dye color and are violet or purple Gram negative bacteria resist the dye color and are red or pink. Gram negative bacteria resist the dye color and are red or pink.

8. Gram Positive Bacteria showing the dark violet or purple color

9. Gram Negative Bacteria showing the red or pinkish color

10. Gram Negative vs. Gram Positive

11. Gram (–) and Gram (+) Bacteria Gram negative Stain: red or pink Outer membrane: present Flagella: 4 rings in basal body Toxins: endotoxins Drying: low resistance Antibody: high resistance Pathogenicity: high (90% of pathogenic bacteria) Gram positive Stain: dark violet or purple Outer membrane: absent Flagella: 2 rings in basal body Toxins: exotoxins Drying: high resistance Antibody: low resistance – more susceptible Pathogenicity: most are not pathogenic

12. Flagellum with Basal Body Rings

13. Bacterial Shapes Bacilli (rod-shaped) Bacilli (rod-shaped) Cocci (sphere-shaped) Cocci (sphere-shaped) Spiral (corkscrew-shaped) Spiral (corkscrew-shaped)

14. Bacteriophages Viruses infecting a bacteria seen here in a electro- micrograph and as plague forming units on a agar plate. Viruses infecting a bacteria seen here in a electro- micrograph and as plague forming units on a agar plate.

15. Timeline of Awareness Van Leeuwenhoek built microscopes and discovered one-celled organisms back in the 1670s. Leeuwenhoek provided science with the knowledge that our world is teeming with small single- celled life. Van Leeuwenhoek built microscopes and discovered one-celled organisms back in the 1670s. Leeuwenhoek provided science with the knowledge that our world is teeming with small single- celled life. Louis Pasteur demonstrated that microorganisms could be grown in broth in sealed tubes – but boiled broth did not grow organisms = Pasteurization ~ 1860s. Louis Pasteur demonstrated that microorganisms could be grown in broth in sealed tubes – but boiled broth did not grow organisms = Pasteurization ~ 1860s. Robert Koch demonstrated that the disease Anthrax was caused by a bacteria ~ 1860s. Robert Koch demonstrated that the disease Anthrax was caused by a bacteria ~ 1860s. Germ theory developed in the 1870s. Germ theory developed in the 1870s.

16. Bacterial Disease Examples Bubonic Plague – Yersinia pestis killed millions of humans, est. 60% of European population Bubonic Plague – Yersinia pestis killed millions of humans, est. 60% of European population E coli O157:H7 – Escherichia coli stain that can cause severe anemia, kidney failure and death E coli O157:H7 – Escherichia coli stain that can cause severe anemia, kidney failure and death Anthrax – Bacillus anthracis produces lasting spores that lethal is breathed or consumed typically in contaminated meat Anthrax – Bacillus anthracis produces lasting spores that lethal is breathed or consumed typically in contaminated meat MRSA – Methicillin-resistant Staphylococcus aureus developed resistance to many antibiotics -widespread in hospitals and clinics MRSA – Methicillin-resistant Staphylococcus aureus developed resistance to many antibiotics -widespread in hospitals and clinics TB – Mycobacterium tuberculosis – attacks lungs, kidneys, spine, and brain – most often fatal. TB – Mycobacterium tuberculosis – attacks lungs, kidneys, spine, and brain – most often fatal.

17. Bacterial Diseases Cholera – Vibrio cholerae infects the intestine through feces contaminated water and food, causes diarrhea, dehydration and death Cholera – Vibrio cholerae infects the intestine through feces contaminated water and food, causes diarrhea, dehydration and death Lyme disease – Borrelia burgdorferi, a tick-borne spirochete causes erythema migrans, arthritis, heart, and nerve damage. Lyme disease – Borrelia burgdorferi, a tick-borne spirochete causes erythema migrans, arthritis, heart, and nerve damage. Tetanus – Clostridium tetani enters body through a break in the skin – puncture wound – a toxin disrupts muscle contraction Tetanus – Clostridium tetani enters body through a break in the skin – puncture wound – a toxin disrupts muscle contraction Gonorrhea – Neisseria gonorrhoeae, a sexually transmitted disease causing inflammation in pelvic and epididymis. Gonorrhea – Neisseria gonorrhoeae, a sexually transmitted disease causing inflammation in pelvic and epididymis.

18. Anthrax, Bacillus anthracis Transmission: Contact of dormant endospores – open skin, inhaled, or ingested. Transmission: Contact of dormant endospores – open skin, inhaled, or ingested. Endospores survive (decades to centuries) are found in soil on every continent including Antarctica Endospores survive (decades to centuries) are found in soil on every continent including Antarctica Hosts are most commonly wild and domestic herbivores – Carnivores become infected consuming infected prey Hosts are most commonly wild and domestic herbivores – Carnivores become infected consuming infected prey Symptoms: pulmonary – flu-like, pneumonia, respiratory collapse (10,000 spores lethal); gastrointestinal – vomit blood, diarrhea, lesions in mouth and throat; Cutaneous – black eschar Symptoms: pulmonary – flu-like, pneumonia, respiratory collapse (10,000 spores lethal); gastrointestinal – vomit blood, diarrhea, lesions in mouth and throat; Cutaneous – black eschar

19. Anthrax – Bacillus anthracis Cutaneous (“skin”) anthrax ulcer with black center (anthracis=Greek anthrakis=“coal” Starts as a boil – ulcer with black center (eschar) then an expanding necrotic ulcer – toxemia - death

20. Anthrax Weapons Biological weapons developed in Russia and US. Concentrated spores into powder used as aerosol Biological weapons developed in Russia and US. Concentrated spores into powder used as aerosol Russian accident or experiment Russian accident or experiment Bioterrorism in US (2001) – concentrated spores placed into envelopes and mailed to news media and two Democratic senators – 22 infected, 5 died. Postal service now scans all mail for anthrax Bioterrorism in US (2001) – concentrated spores placed into envelopes and mailed to news media and two Democratic senators – 22 infected, 5 died. Postal service now scans all mail for anthrax

21. Lyme erythema migrans – Borrelia burgdorferi

22. Plague bubo – Yersinia pestis

23. Tetanus – Clostridium tetani Anaerobic bacteria produces endospores which remain in soil for decades – spores are ubiquitous world wide Anaerobic bacteria produces endospores which remain in soil for decades – spores are ubiquitous world wide Spores reside in the intestines of horses, sheep, cattle, dogs, cats, rats, even chickens and enter soil via manure Spores reside in the intestines of horses, sheep, cattle, dogs, cats, rats, even chickens and enter soil via manure People become infected through a puncture wound or through a cut in the skin People become infected through a puncture wound or through a cut in the skin 2013 ~ 60,000 deaths world wide; 1990 350,000 deaths 2013 ~ 60,000 deaths world wide; 1990 350,000 deaths Tetanus vaccine Tetanus vaccine

24. Tetanus (lockjaw) Tetanus (lockjaw) is caused by the bacterial toxin which interferes with muscle contractions. Tetanus (lockjaw) is caused by the bacterial toxin which interferes with muscle contractions. Muscle spasms start in the jaw then progress through entire body – lasting minutes and occur frequently for weeks to months beginning 3-20 days post exposure Muscle spasms start in the jaw then progress through entire body – lasting minutes and occur frequently for weeks to months beginning 3-20 days post exposure Spasms can be so severe as to cause hyper extension of the body in to a arching position called opisthotonos –the spasms often cause bone fractures Spasms can be so severe as to cause hyper extension of the body in to a arching position called opisthotonos –the spasms often cause bone fractures Mortality with treatment runs ~ 10%. Mortality with treatment runs ~ 10%.