1. Gene nameAcession number C57BL/6 p value MyD88 -/- p value i1/ni1i2/ni2i3/ni3median i1/ni1i1/ni2i3/ni3median Birc2 NM_007465 0.810.920.790.81 0.050 0.781.170.660.780.034 Birc3 NM_007464 1.011.271.15 0.020 0.680.990.570.680.056 Casp1 BC008152 2.462.362.092.36 0.032 1.480.812.511.480.023 Casp8 BC006737 1.461.51.771.5 0.032 1.241.750.981.240.042 Ccl11 AV006589 1.31.241.231.24 0.030 0.941.061.01 0.056 Ccl12 C77789 4.152.793.91 0.045 2.034.012.42 0.032 Ccl2 AF065933 4.542.473.12 0.040 1.452.251.271.450.021 Ccl5 NM_013653 1.060.880.80.88 0.050 1.181.390.471.180.08 Ccl7 AF128193 1.71.561.491.56 0.005 1.061.2811.060.07 Ccl8 NM_021443 1.631.511.041.51 0.050 3.497.741.073.490.0001 Chuk NM_007700 1.221.280.981.22 0.003 1.031370.61.030.07 Cxcl1 NM_008176 3.142.131.652.13 0.027 0.791.272.31.270.02 Cxcl2 NM_009140 1.761.490.911.49 0.015 0.921.211.541.210.04 Erbb2ip BC028256 10.991.31 0.519 0.910.880.480.880.056 Hsp90aa1 C77384 2.882.421.92.42 0.042 0.771.970.760.770.052 Hsp90ab1 BI154147 1.481.451.171.45 0.067 1.31.50.771.30.07 Hsp90b1 BE995678 0.961.460.840.96 0.063 1.982.050.951.980.0032 Ikbkb AF088910 0.70.770.620.7 0.007 1.381.511.081.380.01 Ikbkg NM_010547 1.591.761.62 0.001 0.821.20.9 0.03 Il18 NM_008360 2.031.471.081.47 0.179 0.871.281.03 0.068 Il18bp AF110803 4.094.013.864.01 0.006 0.831.120.87 0.059 Il18r1 NM_008365 1.371.431.191.37 0.066 0.921.760.98 0.02 Il1b BC011437 2.591.922.07 0.001 0.560.794.350.790.024 Il6 NM_031168 2.021.361.51 0.050 0.881.211.581.210.09 Map3k7 D76446 1.991.891,1671.99 0.034 0.981.380.680.980.002 Map3k7ip1 AK009321 1.161.171.061.16 0.271 0.881.210.770.880.056 Map3k7ip2 BM213179 0.941.020.840.94 0.361 0.641.120.550.640.048 Map3k7ip3 AU067719 1.591.661.131.59 0.050 0.620.890.8 0.023 Mapk1 NM_011949 1.731.82 0.016 1.641.760.761.640.001 Mapk10 BI414398 0.960.860.840.86 0.008 0.7410.77 0.045 Mapk11 BM940281 1.381.461.041.38 0.043 0.861.051.01 0.08 Mapk12 BC024514 1.261.470.831.26 0.001 0.651.011.041.010.09 Mapk13 BB453775 1.91.441.69 0.040 0.880.930.92 0.045 Mapk14 AV329330 2.672.532.62 0.041 1.060.790.85 0.034 Mapk3 BC021640 0.820.680.610.68 0.418 0.660.980.66 0.021 Mapk8 NM_011950 1.91.851.491.85 0.003 0.860.910.750.860.056 Mapk9 BC012235 2.362.412.38 0.001 0.871.370.770.870.045 Mefv NM_019453 2.381.561.371.56 0.050 0.861.211.251.210.08 Naip1 AF135491 1.171.551.071.17 0.104 0.51.130.64 0.005 Naip2 NM_010872 1.631.71.391.63 0.052 0.891.130.40.890.054 Naip5 NM_010870 0.680.720.590.68 0.002 0.550.790.480.550.045 Nfkb1 L28118 1.181.331.371.33 0.040 0.831.20.84 0.003 Nfkbia NM_010907 0.930.920.820.92 0.050 0.820.720.78 0.053 Nfkbib NM_010908 1.551.471.421.47 0.038 0.961.191.04 0.0345 Nlrc4 NM_031389 1.381.40.851.38 0.254 0.910.890.780.890.0545 Nlrp3 AF486632 1.681.361.231.36 0.080 1.331.910.951.330.0237 Pstpip1 U87814 0.930.960.890.93 0.457 0.941.230.580.940.04 Pycard (Asc) BG084230 1.761.661.81.76 0.034 0.731.20.73 0.034 Ripk2 NM_138952 1.71.51.011.5 0.049 1.121.630.861.120.06 Sugt1 BE853371 22.442.06 0.006 0.711.230.88 0.043 Tnf NM_013693 2.071.651.631.65 0.097 1.151.31.081.150.01 Tnfaip3 NM_009397 2.561.841.541.84 0.040 1.051.221.11 0.0237 Traf6 BG916928 1.51.421.321.42 0.003 0.91.370.98 0.0467 Trip6 NM_011639 1.271.240.981.24 0.080 0.650.770.630.650.0135 Table S1. DNA microarray during murine malaria The expression value for each gene was determined by calculating the average of differences in intensity (perfect match intensity – mismatch intensity) between its probe pairs. The expression analysis file created from each sample (chip) was imported into GeneSpring 7.2 (Agilent Technologies, Inc., Palo Alto, CA) for further data characterization. A new experiment was generated after importing data from the same organ in which data were normalized by the array to the 50% percentile of all measurements on that array. Lists of genes that were either induced or suppressed between samples from non-infected vs. 6 days infected mice of same genotype were created by filtration-on-fold function within the presented list. The differential expression was evaluated in grouped samples from each mouse genotype (Wt or Knockout mice) and time point (non-infected vs 6 days post infection) using ANOVA. Table S1 - Microarray analysis of P. chabaudi infected mice