1. COLUMN CHROMATOGRAPHY

2. COLUMN CHROMATOGRAPHY
Introduction
Principle
Procedure
Factors
Applications

3. COLUMN CHROMATOGRAPHY
Column chromatography is an extremely valuable technique for purification of synthetic or natural products.
Compounds are separated by CC through the same mechanism as TLC;
Through differential intermolecular forces between the components of the mixture with the mobile phase, and between the components with the stationary phase.

4. COLUMN CHROMATOGRAPHY
Polar components (b) adsorb more strongly to the polar silica and elute after the less polar components (a), which move more quickly with the non-polar (relative to silica) solvent.

5. A variety of adsorbents can be used as the stationary phase;
silica gel ,caco3,celluiose,starch etc. is most commonly used in organic chemistry.
Selection of absorbent

6. Solvent also play important role in this c.c.
Mobile phase
Solvent also play important role in this c.c.
Many types of solvents are available like cyclo hexane, benzene, chloroform, etc.
Selection of solvent

7. Column
The column dimensions are imp for the effective column separation.
Ranges from 1:10 to 1:100
Selection of the column

8. Preparation of column
Dry packing method
Wet packing method.

9. sampling

10. Development technique
Gradient elution
Successive elution
Isocratic elution

16. Factors
Column
Adsorbent
Solvent
Temperature
pressure

17. Applications Separation purification Isolation of active constituents
clinical