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Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 Assembly of a random array 96 well microtiter plate- 1 fibers bundle per well 1 bundle ~50,000.

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Presentation on theme: "Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 Assembly of a random array 96 well microtiter plate- 1 fibers bundle per well 1 bundle ~50,000."— Presentation transcript:

1 Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 Assembly of a random array 96 well microtiter plate- 1 fibers bundle per well 1 bundle ~50,000 glass optical fibers w/ silica bead attached 1,536 bead types exist: yields @ 30-fold redundancy per SNP

2 GoldenGate SNP Genotyping Assay J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004

3 Genotyping data from one bundle J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004

4 Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 Decoding images from eight stages Stage-by-Stage Core-by-Core

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6 Genotype Calling – More difficult SNP

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8 Mean GenCalls Across SNPs


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