1. RESEARCH NEEDS : BIOAVAILABILITY METHODOLOGY

2. IN VITRO METHODOLOGIES Develop simple and reliable methods to screen bioavailability of dietary supplements Validate in vitro methods against human trials

3. In Vivo Methodologies Develop and use reference standards of specific nutrients and bioactive substances in human bioavailability trials Develop methods to measure fractional absorption of nutrients and bioactive substances Determine whether conventional approaches to estimate nutrient bioavailability work for active ingredients of botanicals

4. In Vivo Methodologies - 2 Determine whether “classical drug bioavailability” tests are applicable for bioactive ingredients Develop methods to study absorption/bioavailability of commercial supplements – actual dose levels and tablet forms – in the target population of intended use