1. Recombinant DNA technology 13.2

2. Genetic Engineering  Genetic engineering is a fast more reliable method to increase the frequency of a gene in a population

3.  Recombinant DNA is created by connecting or recombining pieces of DNA from different sources

4. Transgenic organisms  Contain DNA from another (foreign) source

5. Creating recombinant DNA  Restriction enzymes cut both strands of a DNA molecule at a specific nucleotide sequence  This creates pieces or fragments of DNA that contain specific genes

6.  A vector (virus, plasmid, micropipette, etc) transfer the DNA fragment to the new organism, or host

7.  Vectors do not always take the DNA fragments easily, so another enzyme is used to attach the fragments to the vectors

8. Gene cloning  A clone is a genetically identical copy  Gene clones are used for recombinant DNA and other processes such as DNA fingerprinting

9. Polymerase Chain Reaction  This is the process of using an enzyme to make copies of fragments of DNA

10. Applications of DNA technology  What are some applications for recombinant DNA in each of the following areas? Industry Medicine Transgenic animals Agriculture