1. Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription translation

3.  -interferon enhanceosome: Surrounded by two nucleosomes, nuc1 and nuc2. nuc2 covers TATA box and transcription start site. Co-activator GCN5 acetylates nuc1 and nuc2. Acetylation leads to recruitment of co-activators, chromatin remodeling complex, and RNA pol II.

7. Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription translation

8. DNA is restriction digested with restriction enzymes, individually (single-enzyme digest) and in combination (double digest). The restriction fragments are subjected to electrophoresis. The fragments are identified, either using UV absorbing dye or labeled probe. Double digest determines if fragment produced by one enzyme contains restriction sites for the other enzyme. Fragments are aligned by size. Enzyme 1: 8 kb, 6 kb, 3 kb or 3 kb, 6 kb, 8 kb 6 kb, 8 kb, 3 kb or 3 kb, 8 kb, 6 kb 8 kb, 3 kb, 6 kb or 6 kb, 3 kb, 8 kb Enzyme 2: 10 kb, 7 kb or 7 kb, 10 kb Double Digest: 3 kb fragment is split into 2 kb and 1 kb fragments. Restriction Mapping

10. Restriction Fragment Length Polymorphism (RFLP) Individuals can be identified according to RFLP genotype. RFLP locus could be linked to a gene, and thus be used as a diagnostic marker.

11. Use of Restriction Fragment Length Polymorphism I.Marker locus II.Diagnostic A.Medicine B.Forensics III.Assessment of Genetic Variation A.Within and between populations B.Within and between species

12. Restriction Mapping versus RFLP Mapping I.Restriction Mapping A.Based on physical analysis of DNA B.Based on restriction sites with no variation C.Mostly short-range (fine-scale) maps II.RFLP Mapping A.Based on recombination analysis of matings B.Based on restriction-site variation between homologous chromosomes C.Mostly longe-range (coarse-scale) maps

13. Other Useful Approaches 1.Single Nucleotide Polymorphisms (SNPs) Individuals differ in single nucleotides (every 11 to 300 bp in interval). 2.Simple-Sequence Length Polymorphisms (SSLPs) Very short repetitive DNA sequences are more polymorphic than RFLP sequences. These are also called Variable Number Tandem Repeats (VNTRs) - Minisatellite Markers - Microsatellite Markers

14. Simple-Sequence Length Polymorphisms 1.Minisatellite DNA These are 1 to 5 kb in length consisting of repeats 15 to 100 nucleotides in length and are identified by Southern analysis. 2.Microsatellite DNA These are tandem repeats of dinucleotides, commonly stretches of CA. These are identified by gel electrophoresis of PCR products. 5’ C A C A C A C A C A C A C A 3’ 3’ G T G T G T G T G T G T G T 5’