1. The Arabinose Operon Gene Regulation

2. Why Gene Regulation? Developmental Changes Cell Specialization Adaptation to the environment Prevents creation of unneeded product

3. Arabinose Sugar Can be used for food E. coli make three enzymes needed to break down arabinose These enzymes are only made (genes are only expressed) when the arabinose is present.

4. Regulation of the Arabinose Operon The Operon AraBAra AAra D AraC pBAD promoter initiates transcription for all three genes Ara C is a DNA Binding Protein Arabinose AraC RNA Pol P BAD

5. PGLO Plasmid The Operon GFP AraC pBAD promoter initiates transcription Ara C is a DNA Binding Protein GFP is Green Fluorescent Protein Arabinose AraC RNA Pol P BAD

6. References Biotechnology Explorer, pGLO Bacterial Transformation Kit. BioRad.