Presentation is loading. Please wait.

Presentation is loading. Please wait.

Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific.

Published byBryan Brooks Modified over 9 years ago

Similar presentations

Presentation on theme: "Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific."— Presentation transcript:

1

2 Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific sequences CCTTTG AATTCCCAGAATC GGAAACTTAA GGGTCTTAG AATTCGGCCATATACG GCCGGTATATGCTTAA Restriction Sites for EcoRI

3 Electrophoresis Separation of molecules based on size Separation of molecules based on size Negatively charged DNA molecules are pulled through a gel by an electrical field Negatively charged DNA molecules are pulled through a gel by an electrical field Smaller molecules travel faster and farther Smaller molecules travel faster and farther

4 DNA Fingerprinting Variations in DNA Variations in restriction sites Variations in fragment sizes Variations in electrophoresis bands

5 Walter Jordan Poop

6 Gene Cloning Binary Fission Gene Splicing Transformation

7 Mammalian Cloning Somatic cell nuclear transfer

8 Stem Cells

Download ppt "Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific."

Similar presentations

2004-2005 Lab 6: Molecular Biology Description – Gel electrophoresis cut DNA with restriction enzyme fragments separate on gel based on size.

Lab 6: Molecular Biology Description – Gel electrophoresis cut DNA with restriction enzyme fragments separate on gel based on size.
RESTRICTION FRAGMENT LENGTH POLYMORPHISM RFLP. 1. Extraction The first step in DNA typing is extraction of the DNA from the sample, be it blood, saliva,

RESTRICTION FRAGMENT LENGTH POLYMORPHISM RFLP. 1. Extraction The first step in DNA typing is extraction of the DNA from the sample, be it blood, saliva,
Chapter 9: Biotechnology

Chapter 9: Biotechnology
& Gel Plasmid Electrophoresis Mapping.

& Gel Plasmid Electrophoresis Mapping.
Genetic Engineering. We can use a process called gel electrophoresis to separate the pieces.

Genetic Engineering. We can use a process called gel electrophoresis to separate the pieces.
Unit 8 test Biotech study guide.

Unit 8 test Biotech study guide.
DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!

DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!
Biotechnology Technology involving the DNA, genes, and, proteins of different organisms. (Chapter 9) DNA Fingerprinting w/ Gel Electrophoresis Selective.

Biotechnology Technology involving the DNA, genes, and, proteins of different organisms. (Chapter 9) DNA Fingerprinting w/ Gel Electrophoresis Selective.
{ Genetic Engineering Application of molecular genetics (understanding of DNA) for practical purposes.

{ Genetic Engineering Application of molecular genetics (understanding of DNA) for practical purposes.
Biotechnology. Southern Blot/Electrophoresis Electrophoresis- separate DNA with electricity Step 1: DNA is isolated and cut with restriction enzymes –Makes.

Biotechnology. Southern Blot/Electrophoresis Electrophoresis- separate DNA with electricity Step 1: DNA is isolated and cut with restriction enzymes –Makes.
Technological Solutions. In 1977 Sanger et al. were able to work out the complete nucleotide sequence in a virus – (Phage 0X174) This breakthrough allowed.

Technological Solutions. In 1977 Sanger et al. were able to work out the complete nucleotide sequence in a virus – (Phage 0X174) This breakthrough allowed.
Manipulating DNA.

Manipulating DNA.
KEY CONCEPT Biotechnology relies on cutting DNA at specific places.

KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
Genetic Engineering. What is genetic engineering? Application of molecular genetics for practical purposes Used to – identify genes for specific traits.

Genetic Engineering. What is genetic engineering? Application of molecular genetics for practical purposes Used to – identify genes for specific traits.
Ethics of Genetic Engineering Technological Advance Could We? Should We? National DNA registry Prenatal phenotype diagnosis Prenatal disorder correction.

Ethics of Genetic Engineering Technological Advance Could We? Should We? National DNA registry Prenatal phenotype diagnosis Prenatal disorder correction.
NIS - BIOLOGY Lecture 57 – Lecture 58 DNA Technology Ozgur Unal 1.

NIS - BIOLOGY Lecture 57 – Lecture 58 DNA Technology Ozgur Unal 1.
Comparing DNA Aim: How can we compare organisms using gel electrophoresis? Do Now: Write two observations from your results of gel electrophoresis.

Comparing DNA Aim: How can we compare organisms using gel electrophoresis? Do Now: Write two observations from your results of gel electrophoresis.
Restriction Enzyme Analysis of Lambda DNA DNA Fingerprinting of lambda DNA.

Restriction Enzyme Analysis of Lambda DNA DNA Fingerprinting of lambda DNA.
5.3 – Advances in Genetics Trashketball!. Selecting organisms with desired traits to be parents of the next generation is… A. Inbreeding A. Inbreeding.

5.3 – Advances in Genetics Trashketball!. Selecting organisms with desired traits to be parents of the next generation is… A. Inbreeding A. Inbreeding.
DNA Technology.

DNA Technology.

Similar presentations

Ads by Google