1. Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins Trypsin Digestion Glycopeptide Concentration LC-MS Profiling

2. A) B) C) Number of glycopeptide peaks AUC of ROC p-value of t-test mean fold change

3. AUC=0.89 HCC vs HBV HCC HBV HCV Healthy 20 HCC vs HCV AUC=0.86 HCC vs Healthy AUC=0.95

4. MS spectrum MS/MS spectrum Outer fucose SVQEIQATFFYFTPNKTEDTIFLR

6. AUC=0.93 HCC vs HBV HCC HBV HCV Healthy HCC vs HCV AUC=0.86 HCC vs Healthy AUC=0.95 6.5