1. Why synthetic Biology? Reverse Engineering vs. Forward engineering: »Synthetic replicas of natural genetic circuits

3. 1) Why synthetic Biology? Reverse Engineering vs. Forward engineering: »Synthetic replicas of natural genetic circuits »Test sufficiency of design of producing behavior »Done without impairing cellular circuits »Compare different circuit designs that accomplish same function Applications »Nanoscale materials »Cost effective production of anti malaria drugs

4. Lessons learned Noise Computation by simple circuits ????

5. Rewiring signalling pathways. The pheromone and osmolarity response pathways in yeast use protein scaffolds (Ste5 and Pbs2) to avoid crosstalk through the shared component Ste11. By constructing a modified fusion protein of Pbs2 and Ste5, the authors constructed a rewired pathway in which cells produce osmolarity stress responses after pheromone induction

6. 2)Conditions for a Successful Synthetic Biology Well characterized components Modular (analogy to engineering) Orthogonal to cellular processes Robust circuit designs Streamlined process Standardization Decoupling

7. 3) Previous work: focused on transcriptional circuits Principles of noise suppression Bistability Oscillations The benefits of feedback Effect of delays

8. Other mechanisms to design with… Protein Modifications Regulated Degradation microRNA Diffusible molecules ????

9. 4) Who do you think is suited to work in synthetic biology? Biologists? Why? Chemists? Why? Engineers? Why?

10. 5) What is the difference between engineered technological systems and biological systems? Evolution!!!

11. Risks of building synthetic circuits? Thoughts?