1. 1 Introduction to Metabolic Engineering Nils Blüthgen Frank Bruggeman Evangelos Simeonidis Hans V. Westerhoff School of Chemical Engineering and Analytical Science

2. 2 LECTURE CONTENTS Aims Module contents Learning outcomes Timetable Introduction to metabolic engineering in silico

3. 3 AIMS Familiarisation with the integration and regulation of microbial metabolism. Familiarisation with the tools used to study and manipulate metabolic processes in cells.

4. 4 Module contents Introduction Introduction In silico experimentation In silico experimentation Metabolic control analysis Metabolic control analysis General and Thermodynamic aspects of metabolism General and Thermodynamic aspects of metabolism Stoicheiometric aspects Stoicheiometric aspects Metabolic flux analysis Metabolic flux analysis Signal transduction Signal transduction

5. 5 Learning Outcomes Integrate fundamental concepts of biosciences and bioprocess engineering for the study of metabolism (biochemistry, genetic engineering, molecular biology, biology, stoichiometry, thermodynamics, mass balances, calculus) Develop an understanding of the link from genome to cell behaviour/bioreactor performance via metabolome (regulation and control of metabolism, signalling, bioproduct formation)

6. 6 Describe how physico-chemical and biological phenomena interact across spatio-temporal scales (cell biology, compartmentalisation of metabolism, relaxation times) Develop a good appreciation of the multidisciplinary aspects of biotechnology. Learning Outcomes cont…

7. 7 Time Table DateFromTo Folder wit h file sLecturerRoom Wednesday7February11:0013:00 GMSR/F2 Tuesday13February09:0012:00 in silico metabolic engineering (computer practical)MEFeb13FB R/H1 (Sackville G11) Wednesday14February10:0012:00 metabolic control analysis (lecture)MEFeb14FBR/F2 Tuesday20February09:0012:00 metabolic engineering: overview (lecture) with silicon cellMEFeb20HW R/H1 (Sackville G11) Wednesday21February10:0013:00 metabolic engineering: stoichiometric aspects (lecture)MEFeb21HWR/F2 Tuesday6March09:0012:00 flux analysis and flux balance analysisMEMar6ES/NB R/H1 (Sackville G11) Wednesday7March10:0013:00 engineering and signal transductionMEMar7NBR/F2