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Published byDelilah Fleming Modified over 8 years ago
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Transcription Central Dogma Sense (codon) vs antisense (non-coding) Types of RNAs Structural genes vs regulatory genes RNA polymerases (I, II, III) Promoters Transcription factors Mechanism
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The Central Dogma of Biology DNA RNA Protein Transcription Translation Reverse transcriptase
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3 classes of RNA Ribosomal Messenger Transfer 18S(16S) 28S(23S 5S Polycistronic (Bacteria) 4S Components of ribosomes Monocystronic (Eukaryotes)
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A T G C T C T G A C A T A C G A G A C T G T 5’3’ 5’ 3’ BASE COMPLEMENTARITY mRNA A U G C U C U G A C A RED STRAND IS SENSE STRAND GREEN STRAND IS ANTISENSE STRAND SENSE VS ANTISENSE
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Regulator GeneOperator Structural genes ROSG 1 SG 2 SG 3 R-mRNA Polycistronic mRNA Proteins Repressor Repressor-inducer complex Inducer prevents repressor from binding to promoter Basic Model of Gene Regulation in Bacteria Translation
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RNA Polymerase in Bacteriophage T7 Nontemplate (sense strand)
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Control and Enhancer Sequences in Promoter TATA box (Pribnow) GC box CCAAT box Heat shock element Serum response element Glucocorticoid response element Metal response elements Homeodomains
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Promoter Sequences highlighting the TATA box Start Site TATA Box
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Location of Consensus Sequences on Non-template (coding) strand 2 hexlix turns apart
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Pre-transcription Complex
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Factor independent Termination
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Rho-dependent Termination
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