1. Lab Specific Training June 14 th, 2013

2. Responsibilities completing all required safety training; reading the Lab Safety Plan and all lab standard operating procedures; following safety guidelines applicable to the procedures being carried out; assuring that required safety precautions are in place before work is started; following University lab dress code and wearing all PPE required for procedures; Notifying DEHS of accidents, spills or conditions that may warrant further investigation and/or monitoring;

3. Chemical Procedures Hazardous Waste Management -“Hazardous Chemical Waste Management, 5 th edition” * how to handle, label and dispose of hazardous waste -www.dehs.umn.edu

4. Chemical Spills Evacuate -leave the spill area -alert other in the area -Without endangering yourself: remove victims to fresh air, remove contaminated clothing and flush contaminated skin and eyes with water for 15 min -call 911 if anyone has been injured

5. Confine -close door and isolate the area Report -call 911 -DEHS: 6-6002 -DEHS will clean up Secure - post signs and warning tapes

6. Chemicals/ Labels all chemicals should be storage in leak proof bottles and have leak proof secondary container The Chemical Waste Program, (612) 626-1604, hazwaste@tc.umn.eduhazwaste@tc.umn.edu

7. Autoclaving Biological Waste All disposable lab ware, except Pasteur pipettes, contaminated with potentially biohazardous materials. Note: gloves, regardless of contaminated or not, and paper towels used for work surface or equipment decontamination must be disposed of in a clear autoclave bag or red biohazard bag. Culture plates Culture media DO NOT overflow trash, fill it up to ¾ full.

8. All Pasteur pipettes, needles and syringes, broken glass, slides and cover slips, should be placed in sharps containers for pick up. Do not autoclave. Liquid wastes can be decontaminated with one part bleach to ten parts liquid for 30 minutes and then it can go down the drain. Do not add bleach to materials that may contain ammonia or strong acids as dangerous chlorine gas may be produced. As an alternative to autoclaving, contaminated non- liquid materials can be placed in a red Biohazard bag and be collected as Biohazard waste. Red bag disposal costs the University 10X more than disposal of autoclaved waste.

9. Decontamination Work surfaces must be decontaminated with a suitable disinfectant at the end of each day and after any spill of potentially dangerous material. Contaminated or infectious liquid or solid materials must be decontaminated before disposal or reuse. Decontaminate by autoclaving all biologically contaminated materials before washing, reuse or disposal.autoclaving 10% bleach 70% ethanol 10% bleach 70% ethanol

10. Personal Protective Equipments Skin Protection: -long hair should be pulled back -closed toed shoes of non-woven material with non-slip soles -clothing that covers arms and legs -NO SHORTS -lab coats are routine equipment for all lab. workers

11. Gloves: -remove gloves and wash hands before leaving -Inspect before use -clean and discard immediately after use -do not use latex gloves for chemical protection

12. SOPs SOP template can be obtained http://www.dehs.umn.edu/ressafety_rsp_sop.ht m All SOPs should include safety measures that need to occur during each procedures and procedures to followed in the case of any spills, exposures, or accidents All procedures in the lab must have a corresponding SOP.

13. Hygiene Practices eating, drinking and chewing gums are all prohibited before leaving the laboratory, remove personal protective equipment/clothing (lab coat and gloves) and wash hands thoroughly. Do NOT wear laboratory gloves, lab coats or scrubs in public spaces such as hallways, elevators or cafeterias.

14. Please Sign and Date the Sign-In sheet