1. PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING
Advisor: Wei-Shou Hu
Speaker: Justice Akhuamhenkhun
Ace Lai

2. Properties of 1,3-PD Molecular weight 76.09
Viscous and colorless liquid
Miscible with water
Density = g/ cm
MP = -28; BP, between 210 to 212 (celsius degree)
Unsaturated compound with hydroxyl functional group

3. Applications copolyesters, polymers, textile fibres, and carpets
organic solvent ingredient of wood paints and anti-freeze
chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent

4. Why we use bioengineering
Cheaper in cost
Easier in designing pathway
Greener to environment

5. Metabolic pathway

6. Metabolic engineering I
nature of synthesizing of 1,3PD
tpi gene
dhaT and dhaB, gene encoding glycerol dehydratase and PDOR
DAR1, GPP2 encoding 3- phosphate dehydrogenase and glycerol 3-phosphate phosphatase

7. Metabolic engineering II
Redesigning of yqhD
Use of NADPH instead NADH
knock down of tpi to manipulate pathway more efficiently

8. Calculation analysis
Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.

9. Conclusion
DAR1 and GPP2 cloned from Saccharamyces cerevisiae encoding 3- phosphate dehydrogenase
dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase
yqhD from E. coli can produce PDOR as well
tpi manipulated via knockdown to resolve carbon flux
PEP and ATP dependent glucose pathway modified
In all, contribute to higher yield synthesis