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Biomembranes: Structure and Biophysical Properties CHM 6304 section 5731 Spring 2006 meeting time MWF 8th (3-3:50) would like to move meeting to CLB 313.

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Presentation on theme: "Biomembranes: Structure and Biophysical Properties CHM 6304 section 5731 Spring 2006 meeting time MWF 8th (3-3:50) would like to move meeting to CLB 313."— Presentation transcript:

1 Biomembranes: Structure and Biophysical Properties CHM 6304 section 5731 Spring 2006 meeting time MWF 8th (3-3:50) would like to move meeting to CLB 313 (outside my office) Grading: 20% Class Participation 30% In-class Presentations 25% Midterm 25% Final No Classes: Jan 16th Feb 20-24th March 13-17 April 12th

2 Student Presentations (10 registered folks, sign up for 2 - 20 minute talks) Presentations focus on a topic and a technique. Presentations will be on Fridays I as well as the other students will grade you Presentations are based upon literature. You MUST send out a pdf file one week prior to presentation - loss of 20% grade if not. Part of in-class grade will come from questions and discussions generated from each presentation. See syllabus for list of journals. (not inclusive, just a guide)

3 Hopefully lecture will always be ahead of the topics for presentations. Changing of topics is open for discussion, but must first be approved by me.

4 There is no required text book. Recommended Texts: Biomembranes: Molecular Structure and Function Robert B. Gennis The Structure of Biological Membranes Philip L. Yeagle Additional Recommended Texts: The Membranes of Cells Philip L. Yeagle The Phospholipid Manual PLEASE DO NOT RECALL BOOKS FROM LIBRARY I will provide scanned pdf files, material will be supplemented with recent lit and reviews (pdf)

5 To get pdf files: you MUST send me your email address so I can make a list. I will send out email with the link. I will set up a website/ftp site for the course. All materials will be deposited with folders that are dated. Lecture notes will be handed out prior to class. They will NOT be posted on-line.

6 Lipid Structure and Classification amphipathic Polar headgroup Non-polar chains Biomembranes are comprised predominantly of lipids Non-saponifiable Contain fatty acids Do not Contain fatty acids Saponifiable sterols

7 Lipid Classification/Structure Continued

8 OH Fatty acids can be ester or ether linked Phosphoglycerides Sphingosine Ceramide Phosphatydic acid

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10 Note different charges of head groups- learn abbreviations General Structure of Glycerophospholipids

11 Fatty acid diversity saturated cis unsaturated trans unsaturated Oleic acid 9,12-linoleic acid  -linoleic acid  -linoleic acid arachidonic acid Polyunsaturated fatty acids: PUFAs

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14 cardiolipin diacylglycerol dialkylglycerol triacylglycerol trialkylglycerol Cholesteryl oleate Diphosphatidylglycerol DPG Misc. fats (LDL)

15 Sphingolipids Sphingosine N-Acyl-sphingosine or Ceremide Sphingomyelin 1-O-  -D-galactosyl-sphingosine or psychosine

16 Glycolipids Common in plants, rare in animals  -D-Galactosyl-diacylglycerol glycoglycerolipids glycosphingolipids cerebrosides gangliosides sialic acid

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18 Lipopolysaccharides Lipid A Lipid X endotoxin

19 Sterols cholestane

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24 TLC Analysis of Lipids

25 How to make this quantitative? Phosphate Assays...

26 Phospholipase Digestions Followed by GC Analysis


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