Download presentation
Presentation is loading. Please wait.
Published byEdmund Byrd Modified over 9 years ago
1
Biomembranes: Structure and Biophysical Properties CHM 6304 section 5731 Spring 2006 meeting time MWF 8th (3-3:50) would like to move meeting to CLB 313 (outside my office) Grading: 20% Class Participation 30% In-class Presentations 25% Midterm 25% Final No Classes: Jan 16th Feb 20-24th March 13-17 April 12th
2
Student Presentations (10 registered folks, sign up for 2 - 20 minute talks) Presentations focus on a topic and a technique. Presentations will be on Fridays I as well as the other students will grade you Presentations are based upon literature. You MUST send out a pdf file one week prior to presentation - loss of 20% grade if not. Part of in-class grade will come from questions and discussions generated from each presentation. See syllabus for list of journals. (not inclusive, just a guide)
3
Hopefully lecture will always be ahead of the topics for presentations. Changing of topics is open for discussion, but must first be approved by me.
4
There is no required text book. Recommended Texts: Biomembranes: Molecular Structure and Function Robert B. Gennis The Structure of Biological Membranes Philip L. Yeagle Additional Recommended Texts: The Membranes of Cells Philip L. Yeagle The Phospholipid Manual PLEASE DO NOT RECALL BOOKS FROM LIBRARY I will provide scanned pdf files, material will be supplemented with recent lit and reviews (pdf)
5
To get pdf files: you MUST send me your email address so I can make a list. I will send out email with the link. I will set up a website/ftp site for the course. All materials will be deposited with folders that are dated. Lecture notes will be handed out prior to class. They will NOT be posted on-line.
6
Lipid Structure and Classification amphipathic Polar headgroup Non-polar chains Biomembranes are comprised predominantly of lipids Non-saponifiable Contain fatty acids Do not Contain fatty acids Saponifiable sterols
7
Lipid Classification/Structure Continued
8
OH Fatty acids can be ester or ether linked Phosphoglycerides Sphingosine Ceramide Phosphatydic acid
10
Note different charges of head groups- learn abbreviations General Structure of Glycerophospholipids
11
Fatty acid diversity saturated cis unsaturated trans unsaturated Oleic acid 9,12-linoleic acid -linoleic acid -linoleic acid arachidonic acid Polyunsaturated fatty acids: PUFAs
14
cardiolipin diacylglycerol dialkylglycerol triacylglycerol trialkylglycerol Cholesteryl oleate Diphosphatidylglycerol DPG Misc. fats (LDL)
15
Sphingolipids Sphingosine N-Acyl-sphingosine or Ceremide Sphingomyelin 1-O- -D-galactosyl-sphingosine or psychosine
16
Glycolipids Common in plants, rare in animals -D-Galactosyl-diacylglycerol glycoglycerolipids glycosphingolipids cerebrosides gangliosides sialic acid
18
Lipopolysaccharides Lipid A Lipid X endotoxin
19
Sterols cholestane
24
TLC Analysis of Lipids
25
How to make this quantitative? Phosphate Assays...
26
Phospholipase Digestions Followed by GC Analysis
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.