1. Clinical situations where flow cytometry is helpful ­Unexplained cytopenias ­Presence of blasts / atypical cells in blood, marrow or other fluids ­Lymphadenopathy / splenomegaly ­Any extranodal mass suspected to be lymphoma ­Lymphocytosis ­Monocytosis ­Unexplained plasmacytosis ­Monoclonal gammopathies ­Worsening of chronic leukemias or myelodysplasias (NOT very useful in CML in chronic phase) ­Staging for non-Hodgkin’s lymphoma (NOT useful in uncomplicated Hodgkin’s) ­Monitoring of treatment

2. USEFULNESS OF MODERN DIAGNOSTIC TECHNIQUES IN HEMATOLYMPHOID NEOPLASIA DISEASE Microsc IHFCM CG/ FISHPCR Acute leuk. ++ +/- +++ +++ ++ MPD/MDS+++ +/-+/-++++/- Chr.Lymp.Dis. + +/- +++ +/- + Myeloma+++ ++ + +/-+/- NHL +++++++ +/- + Hodgkin’s ++++++ -- - Min. disease + ++ +++ ++ ++++ USEFULNESS OF MODERN DIAGNOSTIC TECHNIQUES IN HEMATOLYMPHOID NEOPLASIA DISEASE Microsc IHFCM CG/ FISHPCR Acute leuk. ++ +/- +++ +++ ++ MPD/MDS+++ +/-+/-++++/- Chr.Lymp.Dis. + +/- +++ +/- + Myeloma+++ ++ + +/-+/- NHL +++++++ +/- + Hodgkin’s ++++++ -- - Min. disease + ++ +++ ++ ++++

3. MODERN DIAGNOSTIC TECHNIQUES METHOD COSTT- A-TIMESENSIT. (%)SAMPLE Microscopy Low minutes (smears) 5 Fresh, frozen 18 hr (tissues) or fixed tissue Immuno-histol. Medium- 6-24 hr 0.1-1 Fresh, frozen highor fixed tissue highor fixed tissue Flow cytometry High3-8 hr0.1-1 Fresh viable tissues or cells CytogeneticsHigh2-10 days 1-5 Fresh viable cells capable of division F.I.S.H. Low-medium18 hr 1-5 Fresh tissues or ce lls PCR Low 24 hr 0.001-1 Any sample MODERN DIAGNOSTIC TECHNIQUES METHOD COSTT- A-TIMESENSIT. (%)SAMPLE Microscopy Low minutes (smears) 5 Fresh, frozen 18 hr (tissues) or fixed tissue Immuno-histol. Medium- 6-24 hr 0.1-1 Fresh, frozen highor fixed tissue highor fixed tissue Flow cytometry High3-8 hr0.1-1 Fresh viable tissues or cells CytogeneticsHigh2-10 days 1-5 Fresh viable cells capable of division F.I.S.H. Low-medium18 hr 1-5 Fresh tissues or ce lls PCR Low 24 hr 0.001-1 Any sample