1. Timothy Johnson Plato Industries, Ltd. Houston, TX
NemOutTM A new nematicide in development for control of reniform and other nematodes in cotton
Timothy Johnson
Plato Industries, Ltd.
Houston, TX

2. Difficulties in managing nematodes in cotton
Multiple species - root knot, reniform, Columbia lance
Soil pests are generally more difficult to manage than foliar pests
Nematodes are difficult to detect and costly to sample
Distributions are typically uneven
Yield losses are often contingent upon additional stress factors

3. Nematode management strategies
Crop rotation – economic and biological limitations
Host plant resistance – slow to develop and bring to market
Chemical and/or biological intervention

4. Nematode management strategies - chemical
Temik® 15G – long time soil insecticide/nematicide
AVICTA® Complete Pak – includes abamectin for nematode control
Vydate® – layby application
Telone® - soil fumigant

5. Nematode management strategies - biological
N-HibitTM – induces plant defense mechanisms

6. NemOutTM Pathogenic fungus Paecilomyces lilacinus strain 251
Primarily an egg parasite with lesser activity on juvenile and adult nematodes

7. NemOutTM
Manufactured by ProPhyta Biologischer Pflanzenschutz GmbH of Poel, Germany
Active ingredient is EPA registered for use on bananas, fruiting vegetables and turf.
NemOut is a new formulation specifically for cotton and peanuts and is being developed by Plato Industries, Ltd. of Houston, TX

8. NemOutTM - mode of action
Paecilomyces lilacinus mycelium infecting nematode eggs.
©Rita Holland

9. NemOutTM – development objectives for cotton and peanuts
Use Rates
Water volumes
Application timing and methodology
Compatibility with fungicides

10. NemOutTM – 2006 field trials
Cotton – Mississippi delta
Peanuts – Alabama and Florida

11. Original 1X formulation
DREC, MSU – Trial 1
Cooperator: Dr. J. Smith
Original 1X formulation
Untreated
2 lbs NemOut IF
4 lbs NemOut IF
8 lbs NemOut IF
Temik 15G 5 lb IF
Avicta C-P
200 ft. plots x 4 rows
6 reps
RCBD
Planted 4/19
Harvested 9/20

12. Measuring Soil Electrical Conductivity (EC)
Veris Data
Trial 1
Measuring Soil Electrical Conductivity (EC)

13. NemOut -Trial 1 Reniform nematodes per 500 cc soil Pre-treatment
Post-treatment

15. NemOutTM – trial 1 harvest
GPS linked picker with continuous read out yield monitor

16. Cooperator: Dr. G. Scuimbato
DREC, MSU – Trial 2
Cooperator: Dr. G. Scuimbato
New 10x formulation
Untreated
0.2 lbs NemOut IF
0.4 lbs NemOut IF
0.8 lbs NemOut IF
Temik 15G 5 lb. IF
Avicta C-P
NemOut Seed Trt.
1 lb/100 CWT
2 lb/100 CWT
2 lb/CWT + Temik
2 lb/CWT + Avicta
45 ft. plots x 4 rows
5 reps
RCBD
Planted 5/4
Harvested 9/26

17. Preparing seed with NemOut
Mixing conidia.
Injecting seed with conidia.
Treated seed.

20. NemOutTM – peanut trial
Cooperator: Dr. J. Rich

21. NemOutTM – peanut trial
Cooperator: Dr. K. Lawrence

22. NemOutTM – future direction
Complete compatibility studies with seed applied fungicides
Expand geographic area for cotton trials to include both root knot and reniform infestations
Focus on application methodologies to include application through irrigation systems and hopper box applications

23. NemOutTM – future direction
Proceed with evaluating use rates that are economically competitive with commercial standards
Complete the registration process to allow large-scale evaluations in 2007

24. Acknowledgements Mississippi State University – DREC Dr. Jim Smith
Dr. Gabe Sciumbato
Dr. Pat English
Boisee Stokes
Troy Gibson
University of Florida – Quincy
Dr. Jim Rich
Auburn University
Dr. Kathy Lawrence