Presentation is loading. Please wait.

Presentation is loading. Please wait.

Molecular basis for the action of Au(I) drugs in rheumatoid arthritis.

Published byGeoffrey Dennis Modified over 9 years ago

Similar presentations

Presentation on theme: "Molecular basis for the action of Au(I) drugs in rheumatoid arthritis."— Presentation transcript:

1 Molecular basis for the action of Au(I) drugs in rheumatoid arthritis

2 Rheumatoid arthritis immune system attacks joints chronic inflammatory disease Wikipedia - Joint, Rheumatoid ArthritisJointRheumatoid Arthritis

3 Anti-inflammatory and immunomodulatory effects of gold(I)-containing drugs J. Biol. Chem., Vol. 276, Issue 36, 34074-34081, September 7, 2001 Collagenase interleukin- 2 (IL-2)IL-2 proteins involved in promoting inflammation Cytokines interleukin-2 receptor genes involved in promoting inflammation down-regulated

4 Anti-inflammatory and immunomodulatory effects of gold(I)-containing drugs J. Biol. Chem., Vol. 276, Issue 36, 34074-34081, September 7, 2001 antioxidative stress proteins  - glutamylcysteine synthetase antioxidative stress genes induce heme oxygenase GO

5 Transcription factors http://en.wikipedia.org/wiki/Transcription_factor http://www.ndsu.edu/instruct/mcclean/plsc731/transcript/transcript1.htm http://www.piercenet.com/Proteomics/browse.cfm?fldID=843944B3-65F9-42A9-BF7A-6F91B4A6FBBB facilitate or inhibit gene expression Regulatory RegionGene Transcription DNA c-Junc-Fos RNA Polymerase II AP-1 transcription factor TGACTCA

6 Gold(I)-drugs inhibit AP-1

7 Electrophoresis EMSA Electrophoretic Mobility Shift Assay Lane 1 is a negative control, and contains only DNA. Lane 2 contains protein as well as a DNA fragment that, based on its sequence, does not interact. Lane 3 contains protein and a DNA fragment that does react; the resulting complex is larger, heavier, and slower-moving. Gel Electrophoresis

8 Gold(I)-drugs inhibit AP-1 binding to DNA Proc. Natl. Acad. Sci. USA Vol. 92, pp. 4497-4501, May 1995

9 Effect of Gold(I)-drugs on other transcription factors Proc. Natl. Acad. Sci. USA Vol. 92, pp. 4497-4501, May 1995

10 Gold(I) inhibition of transcription in vivo Regulatory RegionGene for CAT Transcription DNA c-Junc-Fos RNA Polymerase II AP-1 transcription factor TGACTCA (CAT) chloramphenicol acetyltransferase reporter gene

11 Interaction of Cys residues on Jun and Fos with Au(I) Proc. Natl. Acad. Sci. USA Vol. 92, pp. 4497-4501, May 1995 ~CH 2 SH~CH 2 OH

12 AP-1 : DNA interaction Nature vol. 373, pp.257-261, 1995

13 AP-1 : DNA interaction Nature vol. 373, pp.257-261, 1995

14 AP-1 : DNA interaction Nature vol. 373, pp.257-261, 1995

Download ppt "Molecular basis for the action of Au(I) drugs in rheumatoid arthritis."

Similar presentations

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.
Inhibition of NF-kB by ZAS3, a zinc-finger protein that also binds to the kB motif Presenters: Melissa Sherman & Troy Williams Authors: Joung-Woo Hong,

Inhibition of NF-kB by ZAS3, a zinc-finger protein that also binds to the kB motif Presenters: Melissa Sherman & Troy Williams Authors: Joung-Woo Hong,
Promoters and the Lac Operon. Regulation in Prokaryotes Adjust biochemistry quickly as environment changes Jacob and Monod  extensive studies into the.

Promoters and the Lac Operon. Regulation in Prokaryotes Adjust biochemistry quickly as environment changes Jacob and Monod  extensive studies into the.
Control of Prokaryotic Gene Expression. Prokaryotic Regulation of Genes Regulating Biochemical Pathway for Tryptophan Synthesis. 1.Produce something that.

Control of Prokaryotic Gene Expression. Prokaryotic Regulation of Genes Regulating Biochemical Pathway for Tryptophan Synthesis. 1.Produce something that.
AP Biology Chapter 18: Gene Regulation. Regulation of Gene Expression Important for cellular control and differentiation. Understanding “expression” is.

AP Biology Chapter 18: Gene Regulation. Regulation of Gene Expression Important for cellular control and differentiation. Understanding “expression” is.
RNA Polymerase binding *Constitutive or basal level transcription.

RNA Polymerase binding *Constitutive or basal level transcription.
Cloning and Expression of Transcriptional Repressors in Escherichia coli Sarah-Jane Richards 1 Supervised by Dr. Christophe Corre 2 1. MOAC DTC 2. The.

Cloning and Expression of Transcriptional Repressors in Escherichia coli Sarah-Jane Richards 1 Supervised by Dr. Christophe Corre 2 1. MOAC DTC 2. The.
Bacterial Physiology (Micr430)

Bacterial Physiology (Micr430)
MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two protein 生科系 04 級 賴保諺 891635 Eric Kobet, Xiaoya Zeng,

MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two protein 生科系 04 級 賴保諺 Eric Kobet, Xiaoya Zeng,
MAP Kinase family regulates the nuclear localization of Nrf2 By: Ngan Nguyen Tory Hagen Linus Pauling Institute.

MAP Kinase family regulates the nuclear localization of Nrf2 By: Ngan Nguyen Tory Hagen Linus Pauling Institute.
E. coli RNA Polymerase Sequences of E. coli Promoters.

E. coli RNA Polymerase Sequences of E. coli Promoters.
Introduction Tumor necrosis factor-  (TNF  ) is a pro-inflammatory cytokine important in immune responses TNF  inhibits cAMP-stimulated Cyp17 transcription.

Introduction Tumor necrosis factor-  (TNF  ) is a pro-inflammatory cytokine important in immune responses TNF  inhibits cAMP-stimulated Cyp17 transcription.
Chap. 7 Problem 1 In glucose media without lactose, the lac repressor is bound to the lac operator, and the CAP protein is not bound to its control site.

Chap. 7 Problem 1 In glucose media without lactose, the lac repressor is bound to the lac operator, and the CAP protein is not bound to its control site.
Current Topics in Genomics and Epigenomics – Lecture 2.

Current Topics in Genomics and Epigenomics – Lecture 2.
Gene Expression and Regulation

Gene Expression and Regulation
How Do Biologists Study Gene Regulatory Networks? Journal Club 01/27/05, presented by Hong Lan Introduction to technologies Introduction to HNF4  Go over.

How Do Biologists Study Gene Regulatory Networks? Journal Club 01/27/05, presented by Hong Lan Introduction to technologies Introduction to HNF4  Go over.
RNA-metabolite interactions (riboswitches). RNA aptamers RNA aptamers are structures that bind specifically to target ligands Many aptamers have been.

RNA-metabolite interactions (riboswitches). RNA aptamers RNA aptamers are structures that bind specifically to target ligands Many aptamers have been.
Discovering Macromolecular Interactions. An experimental strategy for identifying new molecular actors in a process candidate approach general screen.

Discovering Macromolecular Interactions. An experimental strategy for identifying new molecular actors in a process candidate approach general screen.
Efficient Bacterial Transcription of DNA Nanocircle Vectors with Optimized Single-Stranded Promoters Tarsuo Ohmichi; Angéle Maki; Eric T. Kool A modular.

Efficient Bacterial Transcription of DNA Nanocircle Vectors with Optimized Single-Stranded Promoters Tarsuo Ohmichi; Angéle Maki; Eric T. Kool A modular.
AP Biology DNA Study Guide. Chapter 16 Molecular Basis of Heredity The structure of DNA The major steps to replication The difference between replication,

AP Biology DNA Study Guide. Chapter 16 Molecular Basis of Heredity The structure of DNA The major steps to replication The difference between replication,

Similar presentations

Ads by Google