1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Human Anatomy & Physiology, Sixth Edition Elaine N. Marieb PowerPoint ® Lecture Slides prepared by Vince Austin, University of Kentucky 17 Blood

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Overview of Blood Circulation  Blood leaves the heart via arteries that branch repeatedly until they become capillaries  Oxygen (O 2 ) and nutrients diffuse across capillary walls and enter tissues  Carbon dioxide (CO 2 ) and wastes move from tissues into the blood

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Overview of Blood Circulation  Oxygen-deficient blood leaves the capillaries and flows in veins to the heart  This blood flows to the lungs where it releases CO 2 and picks up O 2  The oxygen-rich blood returns to the heart

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Composition of Blood  Blood is the body’s only fluid tissue  It is composed of liquid plasma and formed elements  Formed elements include:  Erythrocytes, or red blood cells (RBCs)  Leukocytes, or white blood cells (WBCs)  Platelets  Hematocrit – the percentage of RBCs out of the total blood volume

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Components of Whole Blood Figure 17.1 Withdraw blood and place in tube 1 2 Centrifuge Plasma (55% of whole blood) Formed elements Buffy coat: leukocyctes and platelets (<1% of whole blood) Erythrocytes (45% of whole blood)

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Physical Characteristics and Volume  Blood is a sticky, opaque fluid with a metallic taste  The pH of blood is 7.35–7.45  Temperature is 38  C, slightly higher than “normal” body temperature  Blood accounts for approximately 8% of body weight  Average volume of blood is 5–6 L for males, and 4–5 L for females

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Blood Plasma  Blood plasma contains over 100 solutes, including:  Proteins – albumin, globulins, clotting proteins, and others  Nonprotein nitrogenous substances – lactic acid, urea, creatinine  Organic nutrients – glucose, carbohydrates, amino acids  Electrolytes – sodium, potassium, calcium, chloride, bicarbonate  Respiratory gases – oxygen and carbon dioxide

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Formed Elements  Erythrocytes, leukocytes, and platelets make up the formed elements  Only WBCs are complete cells  RBCs have no nuclei or organelles, and platelets are just cell fragments  Most formed elements survive in the bloodstream for only a few days  Most blood cells do not divide but are renewed by cells in bone marrow

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Erythrocytes (RBCs)  Biconcave discs, anucleate, essentially no organelles  97 % hemoglobin (Hb), a protein that functions in gas transport  Contain the plasma membrane protein spectrin and other proteins that:  Give erythrocytes their flexibility  Allow them to change shape as necessary

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Erythrocytes (RBCs) Figure 17.3

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Erythrocyte Function  Erythrocytes are dedicated to respiratory gas transport  Hemoglobin reversibly binds with oxygen and most oxygen in the blood is bound to hemoglobin  Each heme group bears an atom of iron, which can bind to one oxygen molecule  Each hemoglobin molecule can transport four molecules of oxygen

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Production of Erythrocytes  Hematopoiesis – blood cell formation  Hematopoiesis occurs in the red bone marrow of the:  Axial skeleton and girdles  Epiphyses of the humerus and femur  Hemocytoblasts give rise to all formed elements

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Production of Erythrocytes: Erythropoiesis  A hemocytoblast is transformed into a committed cell called the proerythroblast  Proerythroblasts develop into early erythroblasts  The developmental pathway consists of three phases  Phase 1 – ribosome synthesis in early erythroblasts  Phase 2 – hemoglobin accumulation in late erythroblasts and normoblasts  Phase 3 – ejection of the nucleus from normoblasts and formation of reticulocytes  Reticulocytes then become mature erythrocytes

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Production of Erythrocytes: Erythropoiesis Figure 17.5

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Fate and Destruction of Erythrocytes  The life span of an erythrocyte is 100–120 days  Old erythrocytes become rigid and fragile, and their hemoglobin begins to degenerate  Dying erythrocytes are engulfed by macrophages  Heme and globin are separated and the iron is salvaged for reuse

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Fate and Destruction of Erythrocytes  Heme is degraded to a yellow pigment called bilirubin  The liver secretes bilirubin into the intestines as bile  The intestines metabolize it into urobilinogen  This degraded pigment leaves the body in feces, in a pigment called stercobilin  Globin is metabolized into amino acids and is released into the circulation  Hb released into the blood is captured by haptoglobin and phgocytized

17. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Leukocytes (WBCs)  Leukocytes, the only blood components that are complete cells:  Are less numerous than RBCs  Make up 1% of the total blood volume  Can leave capillaries via diapedesis  Move through tissue spaces  Leukocytosis – WBC count over 11,000 per cubic millimeter  Normal response to bacterial or viral invasion

18. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Leukocytes

19. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Eosinophils account for 1–4% of WBCs  Lead the body’s counterattack against parasitic worms  Lessen the severity of allergies by phagocytizing immune complexes Eosinophils

20. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Account for 0.5% of WBCs and:  Have large, purplish-black (basophilic) granules that contain histamine  Histamine – inflammatory chemical that acts as a vasodilator and attracts other WBCs (antihistamines counter this effect) Basophils

21. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Account for 25% or more of WBCs and:  Are found mostly enmeshed in lymphoid tissue (some circulate in the blood)  There are two types of lymphocytes: T cells and B cells  T cells function in the immune response  B cells give rise to plasma cells, which produce antibodies Lymphocytes

22. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Monocytes account for 4–8% of leukocytes  They are the largest leukocytes  They leave the circulation, enter tissue, and differentiate into macrophages  Macrophages:  Are highly mobile and actively phagocytic Monocytes

23. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Platelets are fragments of megakaryocytes  Platelets function in the clotting mechanism by forming a temporary plug that helps seal breaks in blood vessels Platelets

24. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Genesis of Platelets  The stem cell for platelets is the hemocytoblast  The sequential developmental pathway is hemocytoblast, megakaryoblast, promegakaryocyte, megakaryocyte, and platelets Figure 17.12

25. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  RBC membranes have glycoprotein antigens on their external surfaces  These antigens are:  Unique to the individual  Recognized as foreign if transfused into another individual  Promoters of agglutination and are referred to as agglutinogens  Presence or absence of these antigens is used to classify blood groups Human Blood Groups

26. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  The ABO blood groups consists of:  Two antigens (A and B) on the surface of the RBCs  Two antibodies in the plasma (anti-A and anti-B)  Agglutinogens and their corresponding antibodies cannot be mixed without serious hemolytic reactions ABO Blood Groups

27. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings  Presence of the Rh agglutinogens on RBCs is indicated as Rh +  Anti-Rh antibodies are not spontaneously formed in Rh – individuals  However, if an Rh – individual receives Rh + blood, anti-Rh antibodies form  A second exposure to Rh + blood will result in a typical transfusion reaction Rh Blood Groups