1. MICROSCOPE NOTES

2. MICROSCOPE HISTORY
Developed in the1600’S
Anton van Leeuwenhoek
1st compound microscope
2 lenses (objective,eyepiece)

3. MICROSCOPE TERMS
MAGNIFICATION- To increase the apparent size by means of a lens.
RESOLUTION- Ability to bring object into focus.
FIELD DIAMETER(Field of View)- Area that can be viewed under the microscope.

4. TERMS CONT.
DEPTH OF FIELD- Foreground will come into focus before background.
PARAFOCAL- Object will remain in focus under all objectives.
MONOCULAR- 1 Eyepiece
BINOCULAR- 2 Eyepieces

5. TOTAL MAGNIFICATION
Calculated by multiplying the magnification of the eyepiece by the magnification of the objective.
EX: 10x (eyepiece) X 43x = 430

6. TYPES OF MICROSCOPES
LIGHT MICROSCOPE
ELECTRON MICROSCOPE

7. LIGHT MICROSCOPE
Uses a beam of light passing through one or more lenses to create an enlarged image.

8. Types of Light Microscopes
Bright Field Microscope
Specimen appears dark against a light background

9. COMPOUND LIGHT
Monocular
Magnification up to 450x

10. Stereoscopic Dissecting
Binocular
Lower magnification and greater resolution
Used to view larger specimen

11. ELECTRON MICROSCOPES
Uses a beam of electrons instead of light to form the image.

12. Types of Electron Microscopes
TEM (Transmission electron)
Creates an electron micrograph.
Specimen stained with heavy metal dyes(mercury, arsenic)
Creates 2-D image
Magnifies up to 200,000x

13. Scanning Electron (SEM)
Image viewed on video screen
Not sliced, coated with heavy metal
Creates 3-D image
Magnifies up to 100,000x

14. Scanning Tunneling (STM)
Probe injects electrons into specimen
Computer creates a 3-D image
Can view living specimens
Magnifies up to 50,000x