1. T.T. Liu, fBIRN AHM 2006 Status of Arterial Spin Labeling for the fBIRN Thomas T. Liu UCSD Center for Functional MRI

2. T.T. Liu, fBIRN AHM 2006 Motivation for Arterial Spin Labeling

3. T.T. Liu, fBIRN AHM 2006 Motivation for Arterial Spin Labeling

4. T.T. Liu, fBIRN AHM 2006 Motivation for Arterial Spin Labeling

5. T.T. Liu, fBIRN AHM 2006 Motivation for Arterial Spin Labeling

6. T.T. Liu, fBIRN AHM 2006 Motivation for Arterial Spin Labeling

7. T.T. Liu, fBIRN AHM 2006 Proposed Aims Calibration Aim 3. We will develop and incorporate into the FBIRN imaging protocol a calibration method that adjusts measured BOLD signal based on baseline CBF measured using arterial spin labeling (ASL). Subaim 3.1. Develop, test, and deploy an ASL sequence to the FBIRN sites. Subaim 3.2. Develop and test a calibration algorithm that incorporates baseline CBF measures into the BOLD signal calculation process. Subaim 3.3. Develop, test, and deploy software implementing this calibration algorithm. Subaim 3.4. Incorporate the calibration method into the FBIRN imaging protocol

8. T.T. Liu, fBIRN AHM 2006 Proposed Timeline Year 1: Review available PASL versions and decide on a standard version for FBIRN. Develop an implementation of this standard version on main FBIRN scanner platforms (GE, Siemens). Year 2: Across Development sites, collect data at a range of inversion times to determine transit delays and temporal bolus width (10 patients and 10 controls). Year 3: Using the optimized sequence parameters, measure baseline CBF and the BOLD sensorimotor task in 10 schizophrenic patients and controls at a single site (Series C Step 1). Use these data to develop and test algorithms incorporating baseline CBF measures into the BOLD signal calculation process. Verify across GE and Siemens platforms using retest of the same subjects. Year 4: Further test and refine the calibration method. Develop and field test calibration software. Deploy software and sequences at all FBIRN sites. Collect and process test data at FBIRN sites to verify correct installation and implementation. Year 4.5 and 5: Integrate PASL calibration procedure into FBIRN imaging protocol and use as part of data analysis. Work to make compatible with the BIRN processing pipeline.

9. T.T. Liu, fBIRN AHM 2006 Participating Sites Development Sites GE Sites: UCSD, Stanford Siemens Sites: MGH, Univ. Minnesota Possible Sites for Secondary Group GE Sites: Duke, BWH Siemens Sites: UCI, Yale, Iowa, UNM, UCLA

10. T.T. Liu, fBIRN AHM 2006 UPENN Sequence (at MGH) Single Shot EPI UCSD Sequence Single Shot Spiral FAIRER; TI1/TI2 = 700/1500; 5 mm skip 1; FOV 225mm, matrix 64x64 ; TR 2000ms; 130 reps

11. T.T. Liu, fBIRN AHM 2006 UCSD Sequence Multi-Shot Spiral FOV 220 64x64 5mm skip 1 TR 2.5s TE 3.2ms FAIRER TI1/TI2 600/1500 Time: 6.5 min

12. T.T. Liu, fBIRN AHM 2006 Issues and Tasks 1.Need Siemens research agreement for UMINN 2.Limited technical support for ASL available on Siemens side. Issues Tasks 1.Deploy and test baseline ASL protocol at UCSD, Stanford, UMINN, and MGH. Target coverage: 20 slices; 4 mm skip 1. 2.Decide on whether to include functional ASL protocol

13. T.T. Liu, fBIRN AHM 2006 UPENN Sequence (at MGH) Single Shot EPI (6/8 Partial Fourier) TE 11ms UCSD Sequence Single Shot Spiral; TE 3.2ms FAIRER; TI1/TI2 = 700/1500; 5 mm skip 1; FOV 225mm, matrix 64x64 ; TR 2000ms; 130 reps