1. Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10 th Grade Bellwood-Antis

2. Why I Chose this Experiment Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments. Earlier in the year I had learned a little about Daphnia. I chose this experiment because…

3. Windshield Washer Fluid Contains: Methanol (CHO3H) Antifreeze Contains: Ethylene glycol (C2H6O2) Background Information Brake fluid Contains: Diethylene glycol (C4O3H) Lethal Dose 50 in 96 hours

4. Hypothesis Windshield washer fluid be the least lethal.

5. Why I Chose Daphnia… In our experiment we used the variety of daphnia known as Daphnia Magnum. Daphnia being much smaller, would be more susceptible. Because they are a food source, this could then work its way up the ecosystem. Daphnia are an indicator species.

6. General Procedure 40ml fluid with 4 daphnia 3 trials of each dilution

7. Making a dilution: Ex. 1/10 dilution Water: 34ml Automotive pollutant: 4ml Food:2ml

8. Recipes For My Dilutions DilutionWaterFoodChemical 1/2 18 2 20 1/5 30 2 8 1/10 34 2 4 1/20 36 2 2 1/40 37 2 1 1/80 37 ½ 2 ½

9. Experimental Design Variable ▫the pollutant Experimental Group ▫Antifreeze, Brake Fluid, and Windshield Washer Fluid Control Group ▫Spring Water

10. Experimental Design Contd. Control Variables: Amount of… ▫Light ▫Food ▫Chemical ▫Water Others… ▫Temperature ▫Size of daphnia ▫Clean materials

11. LD 50 Daphnia died too close together

12. Daphnia Lifetime (in minutes) in Brake Fluid

13. Averages Daphnia Lifetimes of 3 Trials Note: All numbers are in minutes.

15. Conclusion According to my data, I reject my hypothesis that Windshield Washer Fluid would be the least lethal to daphnia.

16. Improvements I would… ▫use more specific dilutions. ▫use different animals. ▫use more chemicals.

17. Expanding my Project I could… ▫test motor oil by using a larger aquatic animal. ▫test to see what affect making the water more acidic or basic would have.

18. Bibliography http://ijt.sagepub.com/cgi/content/abstract/23 /1/55 1/24http://ijt.sagepub.com/cgi/content/abstract/23 /1/55 http://www.aehms.org/jaeh_3_2_sherry.html 1/24http://www.aehms.org/jaeh_3_2_sherry.html http://www.jtbaker.com/msds/englishhtml/e51 25.htm 2/8http://www.jtbaker.com/msds/englishhtml/e51 25.htm https://fscimage.fishersci.com/msds/89493.ht m 2/8https://fscimage.fishersci.com/msds/89493.ht m http://www.atsdr.cdc.gov/tfacts96.html 2/10http://www.atsdr.cdc.gov/tfacts96.html