1. COURSE OUTLINE ERT 317Kejuruteraan Biokimia / Biochemical Engineering
Semester 1
2015/2016

2. Nilai unit/ Number of unit: 4
Jenis kursus/ Course type: Teras / Core
Prasyarat/ Prerequisite: ERT 106 Biochemistry
Sinopsis kursus / Course synopsis:
This course focuses on the importance of bioprocess
engineering elements such as enzyme technology,
fermentation and bioconversion technology. Mathematical
representations of enzymatic reaction as well as microbial
systems are featured among lecture topics especially on
kinetics study.

3. Hasil Pembelajaran / Learning Outcomes:
At the end of the course, students are expected to be:
Ability to apply knowledge and solve engineering problem related in developing enzyme reactions based on its kinetics study and applied catalysis.
Ability to identify, formulate, analyze and design solutions complex engineering problems using mathematics principles, natural sciences and engineering sciences for batch and continuous microbial systems, metabolic pathways and kinetics study.
Ability to propose bioconversion technologies of microbial, plant and animal cell culture for the production of value-added product and defend it through verbal presentation in a group.

4. Senarai eksperimen / List of experiments:
Lab 1: To analyze the effect of enzyme and substrate concentration on enzyme kinetics study
Lab 2: To analyze the effect of immobilization method on the retention of enzymatic activity
Lab 3: To analyze the effect of environmental conditions on growth kinetics of S. cerevisae in batch process
Lab 4: Effect of cell immobilization on dye removal by bioabsorbent
Lab 5: To organize an open-ended experiment based on bioconversion technology approaches

5. Pendekatan pembelajaran / Learning approach:
(i) Lecture = 42 hours
(ii) Laboratory = 10 hours
(iii) Tutorial = 12 hours
(iv) PBL = 9 hours

6. Sumbangan penilaian / Evaluation contribution:
Peperiksaan/ Examination: 70 %
Mid Term Examinations = 20 %
Final Examination = 50 %
Penilaian Berterusan/ Continual Assessment: 30 %
Quizzes/Assignment =10 %
Lab report =10 %
Integrated Project =10 %

7. Senarai buku teks dan rujukan / List of text books and references :
Shuler, M. L. and Kargi, F. (2014). Bioprocess Engineering: Basic Concept. 2nd Ed. Upper Saddle River, NJ: Prentice Hall PTR
Rao, D.G. (2010). Introduction to Biochemical engineering. 2nd Ed. Tata McGraw Hill Education Private Limited
Dutta, R. (2008). Fundamentals of Biochemical Engineering. New Delhi: Ane Books India
Katoh, S. and Yoshida, F. (2009). Biochemical Engineering: A Textbook for Engineers Chemists and Biologists. Weinheim: Wiley-VCH Verlag GmbH & Co
Nielsen, J., Villadsen, J. and Gunner L. (2011). Bioreaction Engineering Principles. 3rd Ed. New York: Springer Science+Bussiness Media

8. RANCANGAN MENGAJAR/LESSON PLAN
Minggu/Week
Kandungan Kursus / Course Contents
(Panduan/Guidelines)
Tugasan Bacaan/Reading Assignment
Pensyarah/Lecturer
Week 1 - 2
(7/09 – 18/09)
CHAPTER 1: ENZYME KINETICS AND APPLICATIONS
Chapter 1.1: Kinetics of Enzyme Catalyzed Reactions
Introduction - Classification of enzymes according to the reaction catalyzed and how enzymes work.
Enzyme kinetics - Mechanistic model for simple enzyme kinetics, determination of rate parameters for Michaelis-Menten kinetics; models for more complex enzyme kinetics; effects of pH and temperature and insoluble substrates
Chapter 1
Mrs. Hafiza Shukor
Week 3
(21/09 – 25/09)
Chapter 1.2 : Applied Enzyme Catalysis
Immobilized enzyme systems - Methods of immobilization, diffusional limitations in immobilized enzyme systems, electrostatic and steric effects in immobilized enzyme systems
Enzyme production and application – Large scale production of enzymes, medical and industrial utilization of enzymes
Week 4 - 5
(28/9 – 9/10)
CHAPTER 2: MAJOR METABOLICS PATHWAY
Introduction to metabolism and bioenergetics. Glucose metabolism: Glycolysis and TCA cycle, respiration. Metabolism of nitrogenous compounds and hydrocarbons. Overview of biosynthesis for small and macromolecules, anaerobic and autotrophic metabolism.
Chapter 2
Week 6 - 7
(12/10 – 23/10)
CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES
Chapter 3.1 : Kinetic of Growth in Batch Culture
Quantifying cell concentrations, growth patterns and kinetics in batch culture, effects of environmental conditions on growth kinetics, heat generation by microbial growth.
Chapter 3
Dr. Saleha

9. CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES
Week 8-9
(26/10 – 6/11)
CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES
Chapter 3.2 : Kinetic of Growth in Continuous Culture
Some specific devices for continuous culture, derivation for ideal chemostat, application of chemostat and its deviation of ideality.
Chapter 3
Dr. Saleha
Week 10
(9/11 – 13/11)
Cuti Pertengahan Semester / Mid-semester Break
Week 11 (16/11 – 20/11)
CHAPTER 4: STOICHIOMETRY OF MICROBIAL GROWTH AND PRODUCT FORMATION
Overall growth stoichiometry and design medium formulation, stoichiometric calculation of elemental balances and degree of reduction, theoretical predictions of yield coefficients.
Chapter 4
Week 12-13
(23/11 – 4/12)
CHAPTER 5 : BIOPROCESS CONSIDERATIONS IN USING ANIMAL AND PLANT CELL CULTURES
Chapter 5.1 : Considerations in Using Animal Cell Culture
Structure and biochemistry of animal cells, methods used for the cultivation of animal cells, products of animal cell cultures
(3 hours)
Chapter 5.2 : Considerations in Using Plant Cell Culture
The importance and advantages of plant cell culture, plant cells in culture compare to microbes, methods used for the cultivation of plant cells, economics of plant cell tissue culture
Chapter 5
Week (7/12 – 18/12)
CHAPTER 6: BIOCONVERSION TECHNOLOGIES FOR INDUSTRIAL BIOPROCESSES
Type of bioconversion approaches e.g solid-state and liquid-state, advantages and disadvantages of bioconversion, important factors for bioconversion. Application of microbial, plant and animal cell culture in bioconversion technologies utilizing waste as substrate for production of value-added product.
Chapter 6
Week 16
(21/12 – 25/12)
Minggu Ulangkaji / Revision Weeks
Week
(28/12 – 15/01)
Peperiksaan Akhir Semester/ Final Examination

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