1. Lecture 6 ELISA!

2. E L I S A nzyme- inked mmuno- orbent ssay Protein that facilitates a chemical reaction Immune system - antibodies Survey technique: High throughput Overview

3. Antibodies? Virus H-bonding signature

4. Antibodies? Atrazine How can you use antibodies to detect it?

5. Antibodies? Expose Collect sera Harvest atrazine specific antibodies

6. How ELISA works Competition for the antibody active sites between: Analyte of interest - Antigen (ie. Atrazine) Analyte mimic or antigen mimic

7. The Major Players Compound of interest Atrazine =

8. The Major Players Competitor = E ELISA

9. The Major Players The Antibody ELISA Fe

11. Detection E H2O2H2O2 Compound that is detected BlueYellow via enzyme catalyzed reaction

12. Cross-reactivity AtrazineDesethyl Atrazine Simazine Ametryn