1. Sampling Site N Mariager Fjord Hobro Mariager Kattegat Graphic from http:\\nm.aaa.dk\mfjord Inner fjord salinity: 12-17‰ at top 18-24‰ at bottom Outer fjord salinity: 20-25‰

2. Mariager Catchment Area: 572 km 2 66% agriculture 17% woodland 9% towns 8% lakes Graphic from http:\\nm.aaa.dk\mfjord 1\3 water from catchment

3. Mariager Fjord - Land Reclamation

4. Stratification in the inner fjord - when sampling March 2002 2002 Salinity Oxygen 2002 Graphics from http:\\nm.aaa.dk\mfjord

5. Oxygen Conditions Now Depth Oxygen & Hydrogen Sulphide Oxygen Hydrogen Sulphide Graphic from http:\\nm.aaa.dk\mfjord

6. Oxygen in 1997 Graphic from http:\\nm.aaa.dk\mfjord

7. Oxygen in 1997 Graphic from http:\\nm.aaa.dk\mfjord

8. MOLTEN cores in Mariager Fjord Two sampling occasions September 2001 - MF1-5: possibly over-cored March 2003 - MF6 - 9: intact surface MF8 - Master Core - dated Visual assessment showed no major changes down core - all black & smelly High water content, all levels > 90%

9. Core Correlation MF8, 210 Pb-datedMF6 & MF9 diatom correlated

10. Sediment Properties LOI high (15-30%) Increase at 20cm No major change in particle size

11. Geochemistry - MF2 TN (1-2%), TC (6-15%) & BSi (10-23%) are all high for coastal sediments The changes towards core top will disappear when fluxed/corrected for salt concentration TN TC BSi

12. Pigments - MF6 Comparison between the sites Chlorophyll aCarotenoids Mariager Fjord has a very high concentration of pigments compared to the other long-core sites

13. Mariager Fjord, Denmark No shift in community composition, diatoms and dinoflagellates show species change 1890-1925 Steady increase in chlorophyll a and carotenoids over time Dominating pigments in good agreement with monitored algae (diatoms, dinoflagellates) and the ciliate Mesodinium rubrum Good preservation, low chl a degradation/chl a ratio 2000 1966 1901 1937

14. Cysts from 9 taxa found in core MF9 198519501915 1930 Dinoflagellates 1890

15. Dinoflagellates 1985195019151890 1920 1890 1915 1950 1985 1925

16. 1890 2001 1920 1965 Diatoms Excluded taxa & Benthic:Planktonic excluded

17. Diatoms Main Taxa and Reconstructions

18. Stable Isotopes - MF9 1910 1955 1920 1997 1990 High valuesNo distinct shiftsSeveral possible explanations Increased fertiliser? Periodic anoxia - more denitrification?

19. %N from Stable Isotope Analysis 1910 1997 2000 1990 Decrease in %N at core top - denitrification leading to N release as N 2 gas? Or will it disappear when fluxed/corrected?

20. N Fertilizer applied to Mariager Catchment 1935195519751995 More fertilizer would lead to heavier N isotopes 1000 tons N/year Lag? Graphic from http:\\nm.aaa.dk\mfjord

21. 1890 1920 1975 1993 2000 Fossil fuel burning correction needed - would accentuate increase Productivity proxy?Increase in anoxic events?

22. %C from Stable Isotope Analysis 1890 1920 2000 1993 1975 1997 %C decreases at core top - disappear when fluxed/corrected?

23. Mariager Areal Productivity No obvious change

24. Summation Little change seen in TN, TC & BSi Good pigment preservation, general agreement with monitoring data, but no indication of community shift Largest changes in dinoflagellate species between 1890-1930 Large change in diatom B:P 1890-1920 Transfer functions hampered by lack of analogues in top, little agreement between models and no agreement with monitored data Delta 15N may show fertilizer signal. Delta 13C signal may reflect increase in anoxia