1. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 WP meeting Leafy Vegetables WP 3 Cadot Valérie GEVES : Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences

2. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 A.Objectives  Evaluation for pests and diseases resistance  Evaluation for quality and abiotic characters  Inclusion in the databases to enable a more sustainable production methods and a better quality product

3. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Deliverables for WP3 Deliverable date WP3 Deliverable description StatusPartner responsible Year 1 D16; month 12 Seeds of the selected material of the four crop groups sent to the partners involved On goingP0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13 Year 4 D17; month 45115 accessions of lettuce evaluated Not yet dueP1, P4, P7, P9, P10, P13 D18; month 45320 accessions of spinach evaluated Not yet dueP0, P5 D19; month 45265 accessions of chicory evaluated Not yet dueP2, P8, P12 D20; month 4549 accessions of minor crops evaluated Not yet dueP3, P5, P9, P12 D21; month 45 Evaluation data entered in a fixed format to be sent to the database managers Not yet dueP0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13

4. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Milestones for WP3 Milestone date Milestone descriptionStatusPartner responsible Year 1 month 12 Material selected for the evaluation activities of the four crop groups (see WP1) Done (P12 on going) Pall Year 2 month 18Evaluations started Not yet dueP0, P1, P2, P4, P5, P7, P8, P9, P10, P12, P13 Year 3 month 3670 accessions of lettuce evaluated Not yet dueP1, P4, P7, P9, P10, P13 month 36210 accessions of spinach evaluated Not yet dueP0, P5 month 36170 accessions of chicory evaluated Not yet dueP2, P8, P12 month 3630 accessions of minor crops evaluated Not yet dueP3, P5, P9, P12 Year 4See deliverables Not yet due

5. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 1.What is done by who? a) for pests and diseases resistance  Lettuce: 52 evaluations –Bremia lactucae P4 (CZ), P10 (GBR) –Root-knot nematodes (Meloidogyne sp.) P13 (SLO)  Spinach: 250 evaluations –Downy mildew (Peronospora farinosa) P0 (NLD)  Chicory: 165 evaluations –Thielaviopsis basicolaP2 (FRA) –Alternaria cichoriiP2 (FRA) –Sclerotinia sclerotiorumP2 (FRA)

6. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 1.What is done by who? b) for quality and abiotic characters  Lettuce: 55 evaluations –Post harvest discolorationP1(GBR) –Winter hardinessP7 (AUT) –Quality, taste (organoleptic)P7(AUT) ?, P9 (SLO)  Spinach: 70 evaluations –Nitrate, oxalic acid, dry matterP5 (DAN, SW)  Chicory: 225 evaluations –Shape, color, taste, history, regionalismP8 (CHE) –SesquiterpenesP12 (ITA) –Internal sensory characteristicsP12 (ITA)  Valerianella: 9 evaluations –Winter hardiness, tasteP9 (SLO)  Rucola: 40 evaluations –Nitrate, dry matter P5 (DAN, SW) –Glucosinolate contentP12 (ITA)

7. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P0 (CGN)  What has been done: Start made of discussing the evaluation of spinach accessions with the breeding companies involved.  What will be done: Finalize the plans for the evaluation of spinach accessions.

8. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P1 : started (WHRI) What has been done:  The selected gene bank accessions plus controls were assessed for post harvest discolouration following established experimental protocols.  Significant differences were found between the accessions for mean discolouration. The data is still being analysed as subsets for each day post harvest but so far significant differences have been found between accessions for each day analysed.

9. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P2 (GEVES)  What has been done: it was considered as done for P2 and the material was sent to P8. P12 will ask fresh material from P8 and if necessary, P12 could ask seeds to P2 and P3 when he starts evaluation.  What will be done: Finalize and harmonize with partners involved the scale of notation for evaluation. Coordinate the finalizing of evaluation plans for all the crops with the other leaders of species (P0, P1, P3).

10. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P4 (Palacky)  What has been done: Selection of 30 lettuce accessions for characterization,  In June: Control of viability and pathogenicity of races of Bremis lactucae, pre-selection of isolates to be used for evaluation in a year 2.  What will be done: Evaluation starts in year 2.

11. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P7 (Arche Noah)  What has been done: First selection of accessions to be tested for winter hardiness.  What will be done: Developing criteria for evaluate winter hardiness.

12. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P9 (USOFA)  What has been done: Evaluation for important property and quality for us.

13. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P10 (HDRA)  What has been done: Material for evaluation (lettuce) already selected (all 17 Heritage Seed Library accessions).  What will be done: Nothing planned until year 2

14. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Evaluation for P12 (University of Bologna)  The aspect of sesquiterpenes is still open. In fact most of what was published in literature did not remain in practical. Some delay will occur in the starting of the real evaluation phase, but this will affect only the time development of the program and not the global results.  Bottlenecks: restudy analytical aspect for sesquiterpenes.

15. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Inquiry about the scale of notation for WP3 *Which scale of notation ? * Which data format will be sent? 1. A note with a level of expression associated. Ex : 1= susceptible ; 9 = resistant 2. A measured note. Ex : 50 cm 3. A file linked to the accession number but not advised to sort out 4. A text included in the database as “Remarks” 5. A combination of these 4 possibilities: Indicate which combination you wish. Ex : 5=1+2+4

16. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 SpeciesPartnersPests & Diseases Which scale of notation?Which data format will be sent * (1 to 5) Lettuce P4 (Palacky Univ.) Ales Bremia lactucae For the assessment of sporulation intensity on lettuce cotyledons, a scale 0-3 will be used: 0 = no visible conidiophores on leaves; 1 = limited sporulation, sporadic conidiophores present; 2 = <50% of cotyledon area covered with conidiophores; 3 = >50% of cotyledon area covered with conidiophores. 5 = 1+2+3+4 The data will be presented in: 1) Binary form (+=susceptible, -=resistant); 2) Quantitatively (the final value of sporulation intensity (disease intensity) will be expressed as the percentage of the maximum score); 3) Table will be prepared with these data, i.e. 30 accessions screened by 20 different isolates). P10 (HDRA) Margi Bremia lactucae 1=100% infection 9= 0% infection 5 = 1 + 4 P13 (KIS) Vladimir Nematodes R = resistant (GI < 2, Pf < 1) HR = Hypersensitive reaction (GI > 2, Pf < 1) S = Susceptible (GI > 2, Pf > 1) 1 Spinac h P0 (CGN) Liesbeth Downy mildew, 3-5 races Chicor y P2- SNES Valérie Thielaviopsis basicola R = resistant ; S = Susceptible According statistics: Necrotic Root Index: 0= no symptoms to 4=100% necrotics 5 = 1 + 4 P2- GEVES Valérie Alternaria cichorii R = resistant ; S = Susceptible According statistics: 1=0% infection to 9= 100% infection 5 = 1 + 4 P2-FNPE Valérie Sclerotinia sclerotiorum R = resistant ; S = Susceptible According statistics: classes of necrosis: 0= no symptoms to 5= Chicon wilt 5 = 1 + 4 Scale for pests and diseases : harmonization on going

17. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 [XXX1] For harmonization, it would be better to define 0=absent and 1 = present)[XXX1] [XXX1] For harmonization, it would be better to define 0=absent and 1 = present)[XXX1] Scale for quality and abiotic characters : a few answers 1/2 SpeciesPartnersEvaluationWhich scale of notation?Which data format LettuceP1 (HRI) Dave Post harvest discoloration P7 (Arche Noah) Franziska Winter hardiness 1) 1=no winterhardiness ; 9=complete winter hard 2) % for how much plants survived 3) cm for head measure 4) text for remarks 1+2+4 P7 (Arche Noah) Franziska Taste organoleptic Same scale of notation than P9 P7 (Arche Noah) Franziska Quality Same scale of notation than P91 P9 (USOFA) and P13 (KIS) Mihaela and Vladimir Taste organoleptic P9 (USOFA) and P13 (KIS) Mihaela and Vladimir Quality Type of lettuce (1 = butterhead, 2 = crisphead-batavia, 3 = crisphead-iceberg, 4 = cutting (leaf), 5 = cos (Roman)) Earliness - time of harvest maturity, evaluation 1 to 9 (3 = early, 5 = medium, 7 = late) Beginning of bolting, evaluation 1 to 9 (3 = late, 5 = medium, 7 = early) Plant height in cm (average of 10 plants) Plant width in cm (average of 10 plants) Plant weight in g (average of 10 plants) Plant colour (1 = yellow, 2 = yellow green, 3 = green, 4 = red) Colour intensity, evaluation 1 to 9 (3 = light, 5 = medium, 7 = dark) Anthocyanin coloration (1[XXX1] = present, 2 = absent)[XXX1] Head density, evaluation 1 to 9 (3 = dense, 5 = medium, 7 = loose) Leaf blistering, evaluation 1 to 9 (1 = absent or very weak, 5 = medium, 9 = very strong) Marketable part weight in g (average of 10 plants) Marketable yield (t/ha) (average of 3 repetitions of cca 20 plants) Total yield (t/ha) (average of 3 repetitions of cca 20 plants) 1 [XXX1] For harmonization, it would be better to define 0=absent and 1 = present)[XXX1]

18. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 Scale for quality and abiotic characters : a few answers 2/2 SpeciesPartnersEvaluationWhich scale of notation?Which data format? Spinach P5 (NGB) Katarina Nitrate P5 (NGB) Katarina Oxalic acid P5 (NGB) Katarina Dry matter ChicoryP8 (Pro Specie Rara) Bela Shape, color, taste, history, regionalism 3 ? P12 (UNIBO) Filippo Sesquiterpene lactones external 2 P12 (UNIBO) Filippo Internal sensory characteristics 1 ValerianellaP9 (USOFA) Mihaela Winter hardiness P9 (USOFA) Mihaela Taste (organoleptic) RucolaP5 (NGB) Katarina Nitrate P5 (NGB) Katarina Dry matter P12 (UNIBO) Filippo Glucosinolate content 1

19. V Cadot,: WP meeting Leafy Vegetables 27th September 2007 What about dissemination of results?